Sequences from confirmation amplicon


Confirmation sequences for line 224D12 and hit At4g31330 are shown.
Line ID 224D12
Locus At4g31330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15202184
Primers used for PCR 20kb - 8474, show primer details
   
Sequence Name 66-K113393-0022-224-D12C-8409
GenBank Accession LN511653 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K113393-0022-224-D12C-8409
ATCCTGTCCGGGATAAAAAAATTTGTTTTTTTGAGAAATGACATAAAGGTGGATGGAGAG
GGATGACGTGGCATATATTTGTCCTTTTGTTCGCCATTGTTATTTCCAAAGTGATCTCTC
TCTCTTTTTTTCTTCTTCTTTGTCAACAAGTGATCTCTAGTTGGAGCCCATATCATAGTA
TATATAATTCTTGCACGCTTTTGATTTCTGTCTTCTACCATTTCTTTTGCTTTCTTCTTC
TAGTCATATATATTTGCTTTTTTAAAAGGCCTATGTTAATCATGTATCATGTTTATGTAG
CCGTCAAGTAAAAGTTTATGGTAGGNGACAGAAAT
   
Sequence Name 85-K113394-0022-224-D12C-20kb
GenBank Accession LN514570 [GenBank]
Sequencing primer gene specific primer, 20kb: 5'-ACCATAAACTTTTACTTGACGGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K113394-0022-224-D12C-20kb
CCTTTTAAAAGCAAATTATATGACTAGAAGAAGAAAGCAAAAGAAATGGTAGAAGACAGA
AATCAAAAGCGTGCAAGAATTATATATACTATGATATGGGCTCCAACTAGAGATCACTTG
TTGACAAAGAAGAAGAAAAAAAGAGAGAGAGATCACTTTGGAAATAACAATGGCGAACAA
AAGGACAAATATATGCCACGTCATCCCTCTCCATCCACCTTTATGTCATTTCTCAAAAAA
ACAAATTTTTTTTATCCCGGACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATT

Last Updated on 06.12.2018 16:11