Sequences from confirmation amplicon


Confirmation sequences for line 224G05 and hit At4g14410 are shown.
Line ID 224G05
Locus At4g14410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8301339
Primers used for PCR h104 - 8474, show primer details
   
Sequence Name 49-K015157-022-224-G05C-8409
GenBank Accession LN506659 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K015157-022-224-G05C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGCCGCCGCTGCCGCTT
TGCACCCGGTGGAGCTTGCATGTTGGTTTCTACGCAGAACTGAGCCGGTTAGGCAGATAA
TTTCCATTGAGAACTGAGCCATGTGCACCTTCCCCCCAACACGGTGAGCGACGGGAAACA
TGCCAATGTAGACTTTAACTGTTGTTCTGTCTTCTCTTTATCCGCCTTCAACACCAGCTT
TTCCTCCCTCAGCTCGTTCTTCTCCGCCTATAAAGAAACATACAAACTTAGAAATCTGTT
CACATCCCATTGAACAAGATTCTTGGTTTAGGTGAAAGTGAATGAACAACCAACCTTGAG
ACTCTTGATCTCCTCTAAAAGCTTCTGGTTAGTTTCTTCAAGCTTAAGAGCTTCATCTCT
AAGTTGATTCAATATACGGATTGCATCATCGAGTATAGCCGGTTTATCAGTCTTAGGAGT
CCTCCCAGGCTCCAAAACCGAGCTCAAATCCATAAACCTAACAGAACAACAACAATTTTT
TTCCCCTTGAGACAACTCACTTCACAAAACAAAAACATAGACTAAGCTCTAAACATACCT
CTCATTTAGCTTCTCCCTCCTCAACCTTTCACGACACGCTTTAGTTCCTCCTCCTCTGCT
ACACGATCCAGTCCTTGCCCTATCAACAACCATCACAGATTCAATTAAAAACACAACCAA
CTACCACCAAATCACAAAAAGTTTATATTTTTTCAAAACTCACCGCTTTCTGGAGCAATC
GTCATCTAAATGTTCCTG
   
Sequence Name 53-K015157-022-224-G05C-h104
GenBank Accession LN507978 [GenBank]
Sequencing primer gene specific primer, h104: 5'-GCTGTTAAGGAAGAACAGGAACAT-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K015157-022-224-G05C-H104
GATGACGATTGCTCCAGAAAGCGGTGAGTTTTGAAAAAATATAAACTTTTTGTGATTTGG
TGGTAGTTGGTTGTGTTTTTAATTGAATCTGTGATGGTTGTTGATAGGGCAAGGACTGGA
TCGTGTAGCAGAGGAGGAGGAACTAAAGCGTGTCGTGAAAGGTTGAGGAGGGAGAAGCTA
AATGAGAGGTATGTTTAGAGCTTAGTCTATGTTTTTGTTTTGTGAAGTGAGTTGTCTCAA
GGGGAAAAAAATTGTTGTTGTTCTGTTAGGTTTATGGATTTGAGCTCGGTTTTGGAGCCT
GGGAGGACTCCTAAGACTGATAAACCGGCTATACTCGATGATGCAATCCGTATATTGAAT
CAACTTAGAGATGAAGCTCTTAAGCTTGAAGAAACTAACCAGAAGCTTTTAGAGGAGATC
AAGAGTCTCAAGGTTGGTTGTTCATTCACTTTCACCTAAACCAAGAATCTTGTTCAATGG
GATGTGAACAGATTTCTAAGTTTGTATGTTTCTTTATAGGCGGAGAAGAACGAGCTGAGG
GAGGAAAAGCTGGTGTTGAAGGCGGATAAAGAGAAGACAGAACAACAGTTAAAGTCTACA
TTGGCATGTTTCCCGTCGCTCACCGTGTTGGGGGGAAGGTGCACATGGCTCAGTTCTCAA
TGGAAATTATCTGCCTAACCGGCTCAGTTCTG

Last Updated on 06.12.2018 16:11