Sequences from confirmation amplicon


Confirmation sequences for line 225B03 and hit At5g66560 are shown.
Line ID 225B03
Locus At5g66560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26565063
Primers used for PCR 11uj - 8474, show primer details
   
Sequence Name 48-K117452-0022-225-B03C-11uj
GenBank Accession LN506625 [GenBank]
Sequencing primer gene specific primer, 11uj: 5'-TTGTTGAGGAGATGGAGCTTTTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K117452-0022-225-B03C-11uj
TCTCTGAACTGCGGTTGAAGATGACGATGGCGGTTTCCGGTTAGAACGTAGAATCCCGGG
AATGTGCTTCTTAGCGTAACAAATCAAACAGCTCTCTATGATATCGGAGCTCAGATCTCC
TGATCTCATTGATAGGATCACAGTTTTGAAGATCGGAAGACTCAGTTGCGTCAGATCTTC
AAACCACAGCTCCATGTTCGAATCTCTACTCGGTTTCTTTCGAGATTTCGCCGCGCCGCC
GGGTATAAGTTGCAGATCGGTGGCGGAGATGTTTCCTCTTCCGCCGCCATCGTTTACTGG
CCAACCGAAGAGTGAAGGATCAGCTGAGGAAGCTCTGGAGACTATAGAGTCGATGCACTG
TTCCGTTATACCAAGCGATCCGGCTAAAGGAGAGACTGACTCACAAGCTTTGAGAGCTTT
AATGGATTCACGCAAGCTCTTGTAGACGGAATGAGAGAGAAACCATGTCCGGATATATTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
   
Sequence Name 25-K117451-0022-225-B03C-8409
GenBank Accession LN497634 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K117451-0022-225-B03C-8409
TATATCCGGACTGGNTTTCTCTCTCATTCCGTCTACAGAGCTTGCGTGAATCCATTAAAG
CTCTCAAAGCTTGTGAGTCAGTCTCTCCTTTAGCCGGATCGCTTGGTATAACGGAACAGT
GCATCGACTCTATAGTCTCCAGAGCTTCCTCAGCTGATCCTTCACTCTTCGGTTGGCCAG
TAAACGATGGCGGCGGAAGAGGAAACATCTCCGCCACCGATCTGCAACTTATACCCGGCG
GCGCGGCGAAATCTCGAAAGAAACCGAGTAGAGATTCGAACATGGAGCTGTGGTTTGAAG
ATCTGACGCAACTGAGTCTTCCGATCTTCAAAACTGTGATCCTATCAATGAGATCAGGAG
ATCTGAGCTCCGATATCATAGAGAGCTGTTTGATTTGTTACGCTAAGAAGCACATTCCCG
GGATTCTACGTTCTAACCGGAAACCGCCATCGTCATCTTCAACCGCAGTTTCAGAGAATG
AACAGAGAGAACTACTGGAAACAATAACCTCTAATCTTCCATTAGATAAAAGCTCC

Last Updated on 06.12.2018 16:11