Sequences from confirmation amplicon


Confirmation sequences for line 225F06 and hit At5g27930 are shown.
Line ID 225F06
Locus At5g27930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:9958433
Primers used for PCR 44jx - 8474, show primer details
   
Sequence Name 72-K113549-0022-225-F06C-44jx
GenBank Accession LN512758 [GenBank]
Sequencing primer gene specific primer, 44jx: 5'-GGGTTTTCTGTCTAGATGATGAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K113549-0022-225-F06C-44jx
CCGGGGCTCGCAATGTAAGAGCATTCGGAGACTACTGTATTAAAGAGTATGGATTGGTCT
CAGTCCCTGAAGTCACTCAAAGACACATCTCTACTAAAGACCACTTCATAATCTTGGCCA
GTGATGGGGTAAATACTTATAAACCTTTACACAAACAAAATATACTCATCCACACTCTTA
AAACGTTCAAAAAGTTATAAAAAGTCAATTTGAAAAAGAAAAGAAATAACCACATTTGAA
TAAGAGACTTGGTAATACCAAAAGAAAAGCAAATTGGAATATTGTTTATTTGATTTAGGA
ATTTTTAATCCGAATTTAGGTTTTCCTTGGTTTGAGTTTTTTGGTTTAATGAAAATTACT
CATTGGTATATAAATCTATTATGAATCGACTTGAGTGGTTTTGGTTCGGCTTTGATGTTT
AGGTAGATAGATTTTTAAAGTTCAAATGTATATAAATTTTGGTGTTTGTATACATTTTGG
TTCGATTTGAATCGACTTGGTTTATCTTGGTTGTACAGTCTTAATTAAATTATTTATCGG
CATCTTTAGTAATTAATCTTTTTATTTCTCTTTGGTTTGAATGTAAAAGATATGGGATGT
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 07-K113548w-0022-225-F06C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K113548w-0022-225-F06C-8409
TCAACCAAGAGAATAAAAAGATTAATTACTAAAGATGCCGATAAATAATTTAATTAAGAC
TGTACAACCAAGATAAACCAAGTCGATTCAAATCGAACCAAAATGTATACAAACACCAAA
ATTTATATACATTTGAACTTTAAAAATCTATCTACCTAAACATCAAAGCCGAACCAAAAC
CACTCAAGTCGATTCATAATAGATTTATATACCAATGAGTAATTTTCATTAAACCAAAAA
ACTCAAACCAAGGAAAACCTAAATTCGGATTAAAAATTCCTAAATCAAATAAACAATATT
CCAATTTGCTTTTCTTTTGGTATTACCAAGTCTCTTATTCAAATGTGGTTATTTCTTTTC
TTTTTCAAATTGACTTTTTATAACTTTTTGAACGTTTTAAGAGTGTGGATGAGTATATTT
TGTTTGTGTAAAGGTTTATAAGTATTTACCCCATCACTGGCCAAGATTATGAAGTGGTCT
TTAGTAGAGATGTGTCTTTGAGTGACTTCAGGGACTGAGACCAATCCATACTCTTTAATA
CAGTAGTCTCCGAATGCTCTTGACATTGCGAGCCCCGGTGTTTCTGCGTCTGGCTGCCAC
ACACGATGGACTCCCGGCTCATCATCTAGACGAAAAACC

Last Updated on 06.12.2018 16:11