Sequences from confirmation amplicon


Confirmation sequences for line 225F07 and hit At4g32830 are shown.
Line ID 225F07
Locus At4g32830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15843838
Primers used for PCR AO01 - 8474, show primer details
   
Sequence Name 03-K017883-022-225-F07C-AO01
GenBank Accession LN487276 [GenBank]
Sequencing primer gene specific primer, AO01: 5'-TGGCTAATAAGATCCTTTGCAGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K017883-022-225-F07C-AO01
ATTATTGGTTTGGGAGGGAACTTGAGATCCACTTGCACAATCCTGATATAGATCATTGGT
GGTAACAAATATCATCAAACAAACCCCACAAGAAACAGACTATAATTGTAGATTTCAAAT
GCTGTTTACCTTCTGTATGTGTCTGAGTGCTCCATGGCTTCAAAAGGAGGTACACCATAA
AGAAACTCGTAACAGAGAATCCCAAGGCTCCAGATATCTACACTAGCATCATGTTCTACG
CTTTCGACTGTAAAACAATCCAAAAGGTCACTGTTAAAAAGAATTTAGCAGTGTCCGGGA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGT
TATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAG
GCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC
   
Sequence Name 04-K017883-022-225-F07C-8409
GenBank Accession LN487719 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K017883-022-225-F07C-8409
TAGATTTCCCGGACATGAAGCCATTTCCCGGACACTGCTAAATTCTTTTTAACAGTGACC
TTTTGGATTGTTTTACAGTCGAAAGCGTAGAACATGATGCTAGTGTAGATATCTGGAGCC
TTGGGATTCTCTGTTACGAGTTTCTTTATGGTGTACCTCCTTTTGAAGCCATGGAGCACT
CAGACACATACAGAAGGTAAACAGCATTTGAAATCTACAATTATAGTCTGTTTCTTGTGG
GGTTTGTTTGATGATATTTGTTACCACCAATGATCTATATCAGGATTGTGCAAGTGGATC
TCAAGTTCCCTCCCAAACCAATAATCTCTGCATCTGCAAAGGATCTTANTAGCC

Last Updated on 06.12.2018 16:11