Sequences from confirmation amplicon


Confirmation sequences for line 225F12 and hit At1g67860 are shown.
Line ID 225F12
Locus At1g67860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25444943
Primers used for PCR 16op - 8474, show primer details
   
Sequence Name 56-K114864-0022-225-F12C-16op
GenBank Accession LN509189 [GenBank]
Sequencing primer gene specific primer, 16op: 5'-GACGAACGGAATAAGAACAAACAT-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K114864-0022-225-F12C-16op
AAAAAAGAAAAGATTCTTATCATATCAGGGATATCATATGACGAATAATTGCCATAACAA
ACAAACTTAAGTGAACACTACGAGAATTGTATAAATATATACAAACATCTGAAAGATTGT
AAGCTCTTATTCATATCAAAGAAAAACAACAGAATTAATATATAAAAATGCTTGATACGC
TTATTGGAGGGATTGTCGGAGGGATTGCCGGAGCGATTATGGGAACGGTGGATGGGTTCG
CCAAAGGGATCGGAATATGCCCCGATAGTTACCAAAGCTGCACTCGTACCGACGGCGAGG
AGCACAAAAAGAAGCTCCCGACCAACCTTAGCCGTAACGGCGGTGCACCACCAGTGAAGG
CTAAGGAAAACGGCCGCCGTCCCCGCCAAAAAGACAGGGAGCTAAGGATATATTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCACCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATTTCCGCACG
GTTTATAAAAAA
   
Sequence Name 16-K114863-0022-225-F12C-8409
GenBank Accession LN493540 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K114863-0022-225-F12C-8409
TTACAATTGAATATATCCTTAGCTCCCTGTCTTTCTGGCGGCGACGGCGGCCGTTCTCCT
TAGCCTTCACTGCTGCTGCACCGCCGTTACGGCTAAGGTTGGTCGGGAGCTTCTTTTTGT
GCTCCTCGCAGTCGGTACGAGTGCAGCTCTGGTAACTATCGGGGCATATTCCGATCCCTC
TGGCGAACCCATCCACCGTTCCAATAATCGCTCCGGCAATCCCTCCGACAATCCCTCCAA
TAAGCGTATCAAGCATTTTTATATATTAATTCTGTTGTTTTTCTTTGATATGAATAAGAG
CTTACAATCTTTCAGATGTTTGTATATATTTATACAATTCTCGTAGTGTTCACTTAAGTT
TGTTTGTTATGGCAATTATTCGTCATATGATATCCATGATATGATAAGAATCTTATTCTT
TTTTTTTTACCACCACCATACGTAAAATTGAATAGACTTGTTTGTTCTTATTCCGTTCG

Last Updated on 06.12.2018 16:11