SimpleSearch - Line and FST details
Line specific information
| Line ID | 225F12 |
| Vector Used | pAC161 |
| Line Availability | available as T3 set from NASC (N421576) |
| Segregation Analysis | 50:25:21 |
| Confirmed for Hit | At1g67860 |
| Parent of DUPLO pair | none |
| Parent of pair(s) | 12690 |
Gene hit At1g67860
| Sequence (A. th genome BLAST matches underlined) | >94-K014243-022-225-F12-8409 TTGATCCATGTAGATTCTCCCGTACATGAANGCCTTTACAATTGTAGAAGTTATATTGAA ATGGCTATGATGTGGCCAACAAGTTCTGGTTAACAACTTGCATTATCAATCACATCTTCT TTCATTGTTAATAGGTTTAGCTACATCACCTATGGGATCCTCCCTATAGTGAGAGAGTGC AGCTCTGGTAACTATCGGGGCATATTCCAATCCCTCTGGCGAACCCACCCACCGTTCCAA TAATCGCTCCGGCAATCCCTGCGACTATCCCTCCAATAAGCGCGTCAAGCACTCTTATAT ATTAACCCTGCAGCCCCCCTTGTGATGAGAATAATAGCTCACAATCTTTCACATGTTTGT ATATATTTATACAATCCTCGTAGTGTTCACTTAAGTTTGTTTGCTATGGCAATTATCCGT CATATGATATCCATGATATGATAACAATCTTATTCTC |
| GenBank Accession | AL761463 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr1:25444943 - go to primer design |
| BLAST e Value | 3e-91 |
| Hit Clone Code (BAC ID) | T23K23 |
| Hit Gene Code | At1g67860 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | transmembrane protein |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
Gene hit T2E22
| Sequence (A. th genome BLAST matches underlined) | >94-K014239-022-225-F12-8409 TTGATCCATGTAGATTTGCCCGCACATGAANGCCTTTACAATTGTAGAAGTTATATTGAA ATGGCTATGATGTGGCCAACAAGTTCTGGTTAACAACTTGCATTATCAATCACATCTTCT TTCATTGTTAATAGGTTTAGCTACATCATCTATGGGATCCTCCCTATAGTGAGAGAACGC ACGACTGCGCTACACCCGGGACCCACCCTACACCCAGCCCCGACCCCACCCAACGAACTA ACAATGGCTGCGCCGTTCCCTGA |
| GenBank Accession | AL761462 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr3:3929915 - go to primer design |
| BLAST e Value | 1e-53 |
| Hit Clone Code (BAC ID) | T2E22 |
| Insertion Classification | Genome Hit |
| Confirmation Status | unknown |
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Last Updated on 10.06.2021 13:37 |
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