Sequences from confirmation amplicon


Confirmation sequences for line 225G05 and hit At3g01920 are shown.
Line ID 225G05
Locus At3g01920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:318583
Primers used for PCR GD17 - 8474, show primer details
   
Sequence Name 78-K100816-0022-225-G05C-8409
GenBank Accession LN513644 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K100816-0022-225-G05C-8409
CTTTACAATTGAATATATCCTGTCCACAGTCTCTCTGTAGCTAGTGACGAGATTTTAAAG
AAGAAATAAACCTTGTGCAAATCAATGGGGCATCCATCTGCTGCAGAATAGCTTGGCAGA
GAGCATCATCAGATATGCGGACACCCACGTTTTTCCTAGATGCATATTTCACACTTGTGG
TGCCATAACCTACACACTGTTTGGGTAGTTCCTTGCTTGCAGTCAAGATGAAGGTATACT
GTTAAAAAAAAAAAAAATGCCAAAGATCATTGATAAAAAAACTGTTTGGATATTGCGAAT
ATTTTGAGTATCCTTTAAAAAACAAGAAAAGAAAAGGCTCTCACAGGACCAGGTAAGCAT
TGCTTGACAGCTCGAAAAACGTTAGCATGACCATGACCATCACCACGAGGAAAGCCCATT
GTAAAAGTGTCAATATCTCGAAAGGAACGGCATAAAATGCTA
   
Sequence Name 78-K100828-0022-225-G05C-GD17
GenBank Accession LN513646 [GenBank]
Sequencing primer gene specific primer, GD17: 5'-TATGCAGCCACTTAGCATCTTATG-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K100828-0022-225-G05C-GD17
TTTACATGGGCTTTCCTCGTGGTGATGGTCATGGTCATGCTAACGTTTTTCGAGCTGTCA
AGCAATGCTTACCTGGTCCTGTGAGAGCCTCTTCTTCTCTTGTTTTCTAATGGATACTCA
ACATATTCGCAATATCCAAACAGTTTTCTTATCAATGATCTTTGGCATTTTTTTTTTTTT
TAACAGTATACCTTCATCTTGACGGCAAGCAAGGAACTACCCAAACAGTGTGTAGGTTAT
GGCACCACAAGTGTGAAATATGCATCTGGAAAAAACGTGGGTGTCCCCAAATCTAAAAAT
GCTTTCTGCCAAGCTTTTCTGCAGCAAATGGATGCCCCATTGATTTGCACAAGGTTTATT
TCTTCTTTAAAATCTCGTCACTACCTACAAAAAGACGGTGGACAGGAAATATTCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACTTGGATCACCAATGAGTATAATGGTCAATATGG
AGAAAAAAAAAGAGTAATTACCATTTTTTTTTCATTTCAAAAATGTAAATTTCCCACGTT
TT
Primers used for PCR 14ax - 8474, show primer details
   
Sequence Name 75-K118629-0022-225-G05C-14ax
GenBank Accession LN513306 [GenBank]
Sequencing primer gene specific primer, 14ax: 5'-TAGGTCCCTATACAAACCAACCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K118629-0022-225-G05C-14ax
ATGAGCACAGAATGTTTAGATATATATTACCTTCCCTTCCCTTATGACCTTGGGATATGG
TCCTGTCATATCCACGATGGTGGATGGTTCAGCCACTCGTATACCTCCGTCTACCACAAA
ATCCAGACCCTGTAAAAGGAAACCGAAACAGAGAATAGAGCAACCAAAGATGTTCTTTAG
TCGCTGAGAAAGAGTGAGAGCTATAAATACCTCTGGTCCATATATGTCACCTATTGCGGT
TGGGTCGATCATCCATTCATTTTCTTTAGGCCCTTTCACACTGTCATTATTTACCAATGG
CTTCATGTCCGGACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCACGATTATTAT
   
Sequence Name 56-K118628-0022-225-G05C-8409
GenBank Accession LN509244 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K118628-0022-225-G05C-8409
CATTTACAATTGAATATTCCTGTCCGGACATGAAGCCATTGGTAAATAATGACAGTGTGA
AAGGGCCTAAAGAAAATGAATGGATGATCGACCCAACCGCAATAGGTGACATATATGGAC
CAGAGGTATTTATAGCTCTCACTCTTTCTCAGCGACTAAAGAACATCTTTGGTTGCTCTA
TTCTCTGTTTCGGTTTCCTTTTACAGGGTCTGGATTTTGTGGTAGACGGAGGTATACGAG
TGGCTGAACCATCCACCATCGTGGATATGACAGGACCATATCCCAAGGTCATAAGGGAAG
GGAAGGTAATATATTATCTAAACATTCTGTGCTTCATCCATCCAAGGACCTTAACTTGAC
CGTGTGGTTGGTTTGTATAGGGACCT

Last Updated on 06.12.2018 16:11