Sequences from confirmation amplicon


Confirmation sequences for line 226C09 and hit At2g17890 are shown.
Line ID 226C09
Locus At2g17890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7771275
Primers used for PCR DE07 - 8474, show primer details
   
Sequence Name 03-K019240-022-226-C09C-8409
GenBank Accession LN487285 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K019240-022-226-C09C-8409
AGATTTCCCGGACATGAAGCCATTTACAATTGAATTTACAATTGGCATACTATGTAGCAC
CTGAAGTGTTAAAACGTAGGTCGGGACCTGAATCAGATGTGTGGAGTATTGGTGTAATCA
GTTACATTCTTCTCTGCGGGAGACGACCATTCTGGGATAAGACTGAAGACGGTATCTTCA
AGGAGGTTTGTGTAGTTTCCCTGAAGTAAAAATTCATCAGTAATCGTAAATCTCTAATCA
TGAAGATGTGTTTATAAACATAGGTTTTGAAGAACAAACCTGATTTCAGAAGAAAACCGT
GGCCAACCATTAGCAACAGCGCCAAAGATTTTGTCAAGAAGTTGTTAGTAAAAGACCCGA
GAGCGCGATTAACAGCTGCACAAGCACTATGTAAGCGTTTTGACACCATTTTAAATCATT
TTGTTCTTATCTTTTCTGATCTTTCTGTA
   
Sequence Name 07-K019240-022-226-C09C-DE07
GenBank Accession LN489055 [GenBank]
Sequencing primer gene specific primer, DE07: 5'-TCCACAATCCTTACAGAAAGATCA-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K019240-022-226-C09C-DE07
ATAAGAACTAAATGATTTAAAATGGTGTCAAAACGCTTACATAGTGCTTGTGCAGCTGTT
AATCGCGCTCTCGGGTCTTTTACTAACAACTTCTTGACAAAATCTTTGGCGCTGTTGCTA
ATGGTTGGCCACGGTTTTCTTCTGAAATCAGGTTTGTTCTTCAAAACCTATGTTTATAAA
CACATCTTCATGATTAGAGATTTACGATTACTGATGAATTTTTACTTCAGGGAAACTACA
CAAACCTCCTTGAAGATACCGTCTTCAGTCTTATCCCAGAATGGTCGTCTCCCGCAGAGA
AGAATGTAACTGATTACACCAATACTCCACACATCTGATTCAGGTCCCGACCTACGTTTT
AACACTTCAGGTGCTACATAGTATGCCAATTGTAAATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAA
AGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAA
CAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11