Sequences from confirmation amplicon


Confirmation sequences for line 227B06 and hit At1g06390 are shown.
Line ID 227B06
Locus At1g06390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1949007
Primers used for PCR JC07 - 8474, show primer details
   
Sequence Name 09-K024844-022-227-B06C-8409
GenBank Accession LN490013 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K024844-022-227-B06C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGCATATATTGAGATTTT
GTGGTTTCACATCTCTGTGGCAAACACCGGGAACAGTATGGATGTAAGCCAAACCTCTAA
AGATCTGCACAATAGATATTTCAAATCAGTTTCAGAGTAAATTCTAATAAATTATAATAT
TTGTGAGAACTAACTGCAAATAACAGTACATACTTGATATGTGTAAAGCTTGACATAGAA
AATTGGCATCCTCTGATTAGAACTAGTATAGTGCCTCAAAACCCGGTATAAAGTCTCAGG
TACATACTCCATAACAAGGTTGAGGAAGAGCTCATCTCTACTCGTTGTAGAGAAGAAACA
GTGCTTCAAGGAGATGACATTTGGGTGATCCATTGGTCGCATTAGTTGCAACTCACGGTT
TTTATAGCGTCGATCTTGCAAGACCTTTT
   
Sequence Name 10-K024844-022-227-B06C-JC07
GenBank Accession LN490486 [GenBank]
Sequencing primer gene specific primer, JC07: 5'-CAGTAGCCATTAAAAAGGTCTTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K024844-022-227-B06C-JC07
GACGCTATAAAAACCGTGAGTTGCAACTAATGCGACCAATGGATCACCCAAATGTCATCT
CCTTGAAGCACTGTTTCTTCTCTACAACGAGTAGAGATGAGCTCTTCCTCAACCTTGTTA
TGGAGTATGTACCTGAGACTTTATACCGGGTTTTGAGGCACTATACTAGTTCTAATCAGA
GGATGCCAATTTTCTATGTCAAGCTTTACACATATCAAGTATGTACTGTTATTTGCAGTT
AGTTCTCACAAATATTATAATTTATTAGAATTTACTCTGAAACTGATTTGAAATATCTAT
TGTGCAGATCTTTAGAGGTTTGGCTTACATCCATACTGTTCCCGGTGTTTGCCACAGAGA
TGTGAAACCACAAAATCTCAATATATGCAGGATATATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCCGCGTTATTATAAAATGAA
AGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAGAGCAATAAA
CAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11