Sequences from confirmation amplicon


Confirmation sequences for line 227E02 and hit At4g32920 are shown.
Line ID 227E02
Locus At4g32920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15888974
Primers used for PCR 13xk - 8474, show primer details
   
Sequence Name 29-K119377-0022-227-E02C-13xk
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 13xk: 5'-CTAATGTTCCATGGCTGGAGATAC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K119377-0022-227-E02C-13xk
TGACATATCCCACACAAATGCCACAACCTGCAAAAACCCACCATGCACAAAGGATCTTTA
ATCAAGTAAGAGGAAGTTGCAGAAACTATAGAAGTCGAATTTTCAGATGTAGCTTTTGCA
TAAAGAGAGAAATACTTACGACATTGACCAAGGATGTGACATTCCACAATGCATAAACGC
GTGTGCGACTAGCAGAGCGCCTTGGTCCGTGGCTAAACAAAGCGCTGACCCCAGCAGGGA
ATGGAAAAATGATGCTGAGAGGTAAAATAAACATAGCGAGAAAGACTTCCAGCAATGATA
TCGAGTAGAGTTGGAGTAGTGTGAGTAACGTTAGAGTTAAATCTCCTAGGAGTAGCACCG
AGATAACCAAACCGACCAGATCCTGTTAACACCGCGGAAAATTACAGATTTCAAAACCCT
CAATAGTAAAGGAAAATTACAGATTTCAAAGTCTCAGGGAGACTTTACCTGATGACCAAC
AGGTTTTGTGTTATGAATCAAGAAGGAGATGAGGGAAAGGATATATTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGATTATA
   
Sequence Name 05-K119376-0022-227-E02C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K119376-0022-227-E02C-8409
TTGAATATATCCTTTCCCTCATCTCCTTCTTGATTCATAACACAAAACCTGTTGGTCATC
AGGTAAAGTCTCCCTGAGACTTTGAAATCTGTAATTTTCCTTTACTATTGAGGGTTTTGA
AATCTGTAATTTTCCGCGGTGTTAACAGGATCTGGTCGGTTTGGTTATCTCGGTGCTACT
CCTAGGAGATTTAACTCTAACGTTACTCACACTACTCCAACTCTACTCGATATCATTGCT
GGAAGTCTTTCTCGCTATGTTTATTTTACCTCTCAGCATCATTTTTCCATTCCCTGCTGG
GGTCAGCGCTTTGTTTAGCCACGGACCAAGGCGCTCTGCTAGTCGCACACGCGTTTATGC
ATTGTGGAATGTCACATCCTTGGTCAATGTCGTAAGTATTTCTCTCTTTATGCAAAAGCT
ACATCTGAAAATTCGACTTCTATAGTTTCTGCAACTTCCTCTTACTTGATTAAAGATCCT
TTGTGCATGGTGGGTTTTTGCAGGTTGTGGCATTTGTGTGTGGATATGTTCACTATCATG
GCTCATCATCTGGGAAAAAGATCCCGTATCTCCAGCCATGGAACATTA

Last Updated on 06.12.2018 16:11