SimpleSearch - Line and FST details
Line specific information
| Line ID | 227E02 |
| Vector Used | pAC161 |
| Line Availability | available as T3 set from NASC (N421746) |
| Segregation Analysis | 50:46:44 |
| Confirmed for Hit | At4g32920 |
| Parent of DUPLO pair | none |
| Parent of pair(s) | 5869, 5871, 7729, 8786, 8796, 10449, 10468, 85498, 85499, 85500, 85501 |
Gene hit At4g12070
| Sequence (A. th genome BLAST matches underlined) | >13-K014266-022-227-E02-8409 TTTGATCCATGGTAGATTTCCCGGACATGAAGCTCTTTACAATTGAATATATCCTGTCCC TCATCTCCTTCTTGATTCATAACACAAAACCTGTTGGTCGTCATGGTAAAGTCTCCCTGA GACTTTGAAATCTGTAATTCTCCTTTACTATTGAGGGTTTTGAAATCTGTAATTTTCCGC GGTGTTAGACAGGATCTGGTCGGTTTGGTTCTCTCGGTGCTACTCCTATGGGATCCTCCC TATAGAGTAGTTCAGAATAATGAACAAAAATTGTCGACAAAGTCAATAGATAATTGAAAT GTTTTGGATTTGTGCCGAGGTTTGAGGCTAAACAGAGTAAAATGACAAAGTTTTATGGGC TCTATTCATAACAGTGAGTGGGCTTATGTATAAAGATTTGCAATTGGGATTTTTTTCAAC AAATGTCAAGCGTGATATATGTGTAAATCAACGTCAACTATTTTGTTTAATACTATAAAA TATTGCTTTAAAATGAATCAACGTGTACTTACCTATGGGATCCTCC |
| GenBank Accession | AL761677 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr4:7234326 - go to primer design |
| BLAST e Value | 2e-135 |
| Hit Clone Code (BAC ID) | F16J13 |
| Hit Gene Code | At4g12070 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | hypothetical protein |
| Insertion Classification | TS2TE (3') |
| Confirmation Status | unknown |
Gene hit At3g46000
| Sequence (A. th genome BLAST matches underlined) | >13-K014262-022-227-E02-8409 TTTGATCCATTGTAGATTTCCCGACATGAAGCCCTTTACAATTGAATATATCCTGTTATA TCGATTTTGAAGCTTTCTTCTCGAAAATTTCGATTGCTTGCTATGGGATCCTCCTATAGT GAGACTATGTGAATGACCGATATGCTAATGGTATGGATATACGCACTTGCACGTCACGCG GAGCCTCACTGGAGAGATCACTTCGGTCCTCTCGCTGCTACTACTAGGGTGGAACGTCTT CTTTTTCCACGATGCTCCTCGTGGGTGGGGGTCCATCTTTGGGACCACTGTCGGCAGAGG CATCTTCAACGATGGCCTTTCCTTTATCGCAATGATGGCATTTGTAGGAGCCACCTTCCT TTTCCACTATCTTCACAATAAAGTGACAGATAGCTGGGCAATGGAATCCGAGGAGGCTTC CGGATATTACCCTTTGTTGAAAAGTCTCAATTGCCCTTTGGTCTTCTGAGACTGTATCTC TGATATTCTTGGAGTAAACAAGCGTGTCGTGCTCCACCATGTTGACGAAAATTCTCTTCT TGCCACTGAGTCGNAAGAGACTCTGTATGAACTGCTCCCCCGTCTTTACGGGGAGTTCTG |
| GenBank Accession | FR806128 [GenBank] |
| Graphic View |
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| Predicted Position of Insertion | Chr3:16908784 - go to primer design |
| BLAST e Value | 2e-15 |
| Hit Clone Code (BAC ID) | F16L2 |
| Hit Gene Code | At3g46000 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | actin depolymerizing factor 2 |
| Insertion Classification | CDSi |
| Confirmation Status | unknown |
Gene hit At4g32920
| Sequence (A. th genome BLAST matches underlined) | >13-K014266-022-227-E02-8409 TTTGATCCATGGTAGATTTCCCGGACATGAAGCTCTTTACAATTGAATATATCCTGTCCC TCATCTCCTTCTTGATTCATAACACAAAACCTGTTGGTCGTCATGGTAAAGTCTCCCTGA GACTTTGAAATCTGTAATTCTCCTTTACTATTGAGGGTTTTGAAATCTGTAATTTTCCGC GGTGTTAGACAGGATCTGGTCGGTTTGGTTCTCTCGGTGCTACTCCTATGGGATCCTCCC TATAGAGTAGTTCAGAATAATGAACAAAAATTGTCGACAAAGTCAATAGATAATTGAAAT GTTTTGGATTTGTGCCGAGGTTTGAGGCTAAACAGAGTAAAATGACAAAGTTTTATGGGC TCTATTCATAACAGTGAGTGGGCTTATGTATAAAGATTTGCAATTGGGATTTTTTTCAAC AAATGTCAAGCGTGATATATGTGTAAATCAACGTCAACTATTTTGTTTAATACTATAAAA TATTGCTTTAAAATGAATCAACGTGTACTTACCTATGGGATCCTCC |
| GenBank Accession | AL761677 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr4:15888974 - go to primer design |
| BLAST e Value | 2e-76 |
| Hit Clone Code (BAC ID) | F26P21 |
| Hit Gene Code | At4g32920 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | glycine-rich protein |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
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Last Updated on 10.06.2021 13:37 |
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