Sequences from confirmation amplicon


Confirmation sequences for line 227H12 and hit At2g21230 are shown.
Line ID 227H12
Locus At2g21230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9096301
Primers used for PCR 08oh - 8474, show primer details
   
Sequence Name 70-K114864-0022-227-H12C-08oh
GenBank Accession LN512438 [GenBank]
Sequencing primer gene specific primer, 08oh: 5'-TCAACACCTATGAGTTCAATCAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K114864-0022-227-H12C-08oh
TCAAGAGCATGCAATGTCACGAGTTTAATCATGAGCAACCAAACCATGAATTTTATGGAC
ATAACTAAAGGATTAAACATGTCTTCAAGTTGTTTTAGATTTATCTATATATGATTATAA
ATTTGTAACCGTGAGAGGGTTCAGACAATATGATTTTTTTTTAATATTTTTGTTTAGTTT
ATTTCATTAAAGCTTTGTTCTTTCATTTTTTTGTTATTGAGATTAATTTTTATTTTATCA
AATTTGTTGAATTTGATAGAGAAATAAACATACTCTATATGTAATTAGACGAAAGTAGTC
TTTTCCGACCAGTGGATGATTGCTGCTTTGGTCAACGTTTCAAAAGAATCGTTTTTTCTA
TATGATATATAGTGAATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 30-K114863-0022-227-H12C-8409
GenBank Accession LN499840 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K114863-0022-227-H12C-8409
ATTTACATTGAATATATCCTGATTCACTATATATCATATAGAAAAAACGATTCTTTTGAA
ACGTTGACCAAAGCAGCAATCATCCACTGGTCGGAAAAGACTACTTTCGTCTAATTACAT
ATAGAGTATGTTTATTTCTCTATCAAATTCAACAAATTTGATAAAATAAAAATTAATCTC
AATAACAAAAAAATGAAAGAACAAAGCTTTAATGAAATAAACTAAACAAAAATATTAAAA
AAAAATCATATTGTCTGAACCCTCTCACGGTTACAAATTTATAATCATATATAGATAAAT
CTAAAACAACTTGAAGACATGTTTAATCCTTTAGTTATGTCCATAAAATTCATGGTTTGG
TTGCTCATGATTAAACTCGTTGACATTGCATTGCTCTTTGAATATGTTTGGGTCCAATTG
CTGGTGCTGATTGAACTCATAGGTGTTGA

Last Updated on 06.12.2018 16:11