Sequences from confirmation amplicon


Confirmation sequences for line 228A07 and hit At5g03840 are shown.
Line ID 228A07
Locus At5g03840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1025516
Primers used for PCR MR68 - 8474, show primer details
   
Sequence Name 10-K017365-022-228-A07C-MR68
GenBank Accession LN490429 [GenBank]
Sequencing primer gene specific primer, MR68: 5'-GCAAATTTACGAGTGTTGAAGTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K017365-022-228-A07C-MR68
GGATATTACGGAAAGATAACACGTCTTTGCTTCTGCCTGAACAGAACAAACACAAACCTA
TGTATCCCTATGCTTGGCCTTGGCAATTCATAGCTCACCACCTCTTTGCCTATATTACAA
TGCAAATATTTTTATTTCTATATAACATGATATATGTATAAGTATTTAAATTACAAGATT
CATGAAGAGGCCTTACCAAACGTAGCATCTGTTGTGCCGGGAATGTTTGTAACGATCCTA
TTGAAACATTCAACAATACTAATATTATTACTTTACAGAAAAAGTTCTGAGAACTCATGG
CGATGGAGATCTTTGACCAACTCAAATGTATATAACTTACACCCAAAAAAAACTTTATTA
GGGTTATAACGAAATGATTTAAATGCAATGTATATGGAATATGGGCCCAAAATGAAAAGA
AAGAATAAATAAATTAAACGTACCAGTGCAGGTGTTCTTTTAGAAAGGGGTCACTAGGAC
CTGGAACATCTGGGTCTATCATCACCTGTTTTGCATTTCGGTTGAGTTTTACCATTAAAA
AAAAAAGACTGGAAAAAGGAATGATAAAAACTAAAGCTACAATTTATAAGATTATTGAGT
TAGAAATGGAAAACTTGTAAGAGGAAAAGGAAGTGAGACGTTTTAATGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
   
Sequence Name 14-K017365-022-228-A07C-8409
GenBank Accession LN492285 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K017365-022-228-A07C-8409
AGATTTCCCCGACATGAAGCCATTTACATTAAAACGTCTCACTTCCTTTTCCTCTTACAA
GTTTTCCATTTCTAACTCAATAATCTTATAAATTGTAGCTTTAGTTTTTATCATTCCTTT
TTCCAGTCTTTTTTTTTTAATGGTAAAACTCAACCGAAATGCAAAACAGGGGATGATAGA
CCCAGATGTTCCAGGTCCTAGTGACCCCTTTCTAAAAAAACACCTGCACTGGTACGTTTA
ATTTATTTATTCTTTCTTTTCATTTTGGGCCCATATTCCATATACATTGCATTTAAATCA
TTTCGTTATAACCCTAATAAAGTTTTTTTTGGGGGTAAGTTATATACATTTGAGTTGGTC
AAAGATCTCCATCGCCATGAGTTCTCAAAACTTTTTCTGTAAAGTAATAATATTAGTATT
GTTGAATGTTTCAATAGGATCGTTACAAACATTCCCGGCACAACAGATGCTACGTTTGGT
AAGGCCTCTTCATGAATCTTGTAATTTAAATACTTATACATATATCATGTTATATAGAAA
TAAAAATATTTGCATTGTAATATAGGCAAAGAGGTGGTGAGCTATGAATTGCCAAGGCCA
AGCATAGGGATACATAGGTTTGTGTTTGTTCTGTTCA

Last Updated on 06.12.2018 16:11