Genotyping and primer details

Genotyping and primer details for 228A07 - At5g03840
Line ID 228A07
Locus At5g03840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:1025516 of line 228A07 Chr5:1025516
Insertion allele amplicon 8474 - MR68
Locus specific primer MR68: 5'-GCAAATTTACGAGTGTTGAAGTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:1024839
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11