Sequences from confirmation amplicon


Confirmation sequences for line 228A08 and hit At4g09990 are shown.
Line ID 228A08
Locus At4g09990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:6260227
Primers used for PCR 06ww - 8474, show primer details
   
Sequence Name 45-K119110-0022-228-A08C-06ww
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 06ww: 5'-TTGTTGGAATCTTTCACTTTTGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K119110-0022-228-A08C-06ww
GGATTTCTTAGTACTGTCTCAATCCAAGCTTCATCTTCCTCGAGGAACACTAGCAATGTA
AGAAGTAACTTTATAAATGAAAAACAGAGATATGATTAATCTTTATAGATCTGTTGTTAT
GAAAAGCTTACAAGTGCGTCCACCGTGATTGAGAGATGCCCACATCAAGCTATCGTGTCC
TAAGCCAAAGACGAGGAAGTTACAAGGAGACTTCTTGTCCAAGACTCTCTTTGAGACTGA
GACTTCATCAAACGTTTGTTGTGGTGTGATCTCTGAGGTGACATAGTGAACCAATGCGTC
GGATAGAGAAGTGGGCAATTTGGTGCATTGTTGTGGGGTTGATGGGCACTTCGATTTCTG
GTGATATTCGTCTCGAATGGTTGCGGTTGAATTGGAAGAGAAGCTTGCTCTTTTCAAGAA
GAGAATGAACAACACCAAGATTGAGCAACAAATGAATATAAGCTTTGAGCTGATGAATGA
TTGGGATTTATTCCTCATGTTTTCCTTTCTCTCTCGGTTTTAGCGTTTTCGATATGTTTT
GACAATGGTGAAGATGGTGAGAGATAGTCTTGTGGGTGAATAGATGCATCCTTAAAGAGA
GAATGATTCTGGAAGTTGAGGATGACATGACGGCGACTAAGGTTGCTGTTGAACCAGAAG
TTGGTGGATCTACATGTAAATATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAAATTCAAAATGTAGATGTCCG
   
Sequence Name 22-K119109-0022-228-A08C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K119109-0022-228-A08C-8409
CACTTCTGGTTCACAGCAACCTTAGTCGCCGTCATGTCATCCTCAACTTCCAGAATCATT
CTCTCTTTAAGGATGCATCTATTCACCCACAAGACTATCTCTCACCATCTTCACCATTGT
CAAAACATATCGAAAACGCTAAAACCGAGAGAGAAAGGAAAACATGAGGAATAAATCCCA
ATCATTCATCAGCTCAAAGCTTATATTCATTTGTTGCTCAATCTTGGTGTTGTTCATTCT
CTTCTTGAAAAGAGCAAGCTTCTCTTCCAATTCAACCGCAACCATTCGAGACGAATATCA
CCAGAAATCGAAGTGCCCATCAACCCCACAACAATGCACCAAATTGCCCACTTCTCTATC
CGACGCATTGGTTCACTATGTCACCTCAGAGATCACACCACAACAAACGTTTGATGAAGT
CTCAGTCTCAAAGAGAGTCTTGGACAAGAAGTCTCCTTGTAACTTCCTCGTCTTTGGCTT
AGGACACGATAGCTTGATGTGGGCATCTCTCAATCACGGTGGACGCACTTGTAAGCTTTT
CATAACAACAGATCTATAAAGATTAATCATATCTCTGTTTTTCATTTATAAAGTTACTTC
TTACATTGCTAGTGTTCCTCGAGGAAGATGAAGCTTGGATTGAGACAGTAACTAAGAAAT
TCCCAAACTTGGAATCCTACCACGTCGTATACGACACANAAGTGAA

Last Updated on 06.12.2018 16:11