Sequences from confirmation amplicon


Confirmation sequences for line 228E12 and hit At3g56760 are shown.
Line ID 228E12
Locus At3g56760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21020657
Primers used for PCR 10qp - 8474, show primer details
   
Sequence Name 26-K115781-0022-228-E12C-10qp
GenBank Accession LN498069 [GenBank]
Sequencing primer gene specific primer, 10qp: 5'-CTAGACTTTGAAGAGTTTTGCGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K115781-0022-228-E12C-10qp
CATGGAGACTTGGGAGCACATGCACGCCGTGCATATGAGTTGTATGAGAAGGATGGGAAC
AGAGTCATCATGATCGAAGAACTTGCCACGGTATAAAACTTCTCTTCGAGATGTGATATC
ACCGATGTCTTTGGTTGGCGCTTGATGCGTTTGTGCATATGTGTTGTTTTGTTGTTGACA
GGAACTCGGACTAGGTCCATCGGTGCCTGTTCATGTTGTTCTTCAAGATTGGATAAGACA
TTCTGATGGGAAGCTAAGTTTCTTGGGTTTTGTCAGACTCTTACACGGCGTCTCCTCTCG
TACATTGCAGAAAGCGTAGAAGAGAATTAGATTAAAGATTTGAAAAAAGAAACAAAAGAA
AAAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCG
   
Sequence Name 02-K115780-0022-228-E12C-8409
GenBank Accession LN487173 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K115780-0022-228-E12C-8409
TCTTCTACGCTTCNGCAATGTACGAGAGGAGACGCCGTGTAAGAGTCTGACAAAACCCAA
GAAACTTAGCTTCCCATCAGAATGTCTTATCCAATCTTGAAGAACAACATGAACAGGCAC
CGATGGACCTAGTCCGAGTTCCTGTCAACAACAAAACAACACATATGCACAAACGCATCA
AGCGCCAACCAAAGACATCGGTGATATCACATCTCGAAGAGAAGTTTTATACCGTGGCAA
GTTCTTCGATCATGATGACTCTGTTCCCATCCTTCTCATACAACTCATATGCACGGCGTG
CATGTTGCTCCCAAGTCTCCATTGCTTCTAGCTGATATACACTTAAAGCTGAAGCGCAAA
ACTCTCAAAAGTCTA

Last Updated on 06.12.2018 16:11