Sequences from confirmation amplicon


Confirmation sequences for line 228F07 and hit At5g40100 are shown.
Line ID 228F07
Locus At5g40100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16044923
Primers used for PCR 18lm - 8474, show primer details
   
Sequence Name 64-K114129-0022-228-F07C-18lm
GenBank Accession LN511252 [GenBank]
Sequencing primer gene specific primer, 18lm: 5'-TCTCTGCTAAAACTCTCATCCACA-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K114129-0022-228-F07C-18lm
GTGACACGCCGGAAGTGTCTCCATTAAAGAGACATGCAACATGGAGAAAAGCATTCTGAT
GTGCTTTTGCTAAACCCTCGTAGCTAATTTTCAGGATTTCCATTATATTCTCGTCAGGAG
TACTTTCAAGTCCACATACTGCTTCTTCCCATTCTTCAGGAGAGTTGGCCCTTCCACGGA
GAAATAAGGCATAGGCTTGAAGGGCAGAAGGAAGCCCGTGCGCAAGCCGAGCTGCTCGGA
TTGAGAGCTGTTCAAAATCAACAGAAGGAGGACTGCCTCCTTCAAAAGCAATCTGTTTAA
ACATCTGCAGGGAATCCTTGTCGTCCAAGCACTTAACATCATAGATGGTTTTTACTCCAC
AACTATNGAGCAAGCCCTTGTCTCGGGTGGTTATGATGATTCGGCTCTGACGACCAAACC
ATCTTGTCTCTTTTGCCAGAGCATGGATCTGTACCAGTTTATCCACACCATCAAGCACAA
GAAGAACCTTGTGGTTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 22-K114128-0022-228-F07C-8409
GenBank Accession LN496205 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K114128-0022-228-F07C-8409
TCGGACACAGGTTCTTCTTGTGCTTGATGGTGTGGATAACTGGTACAGATCCATGCTCTG
GCAAAAGAGACAAGATGGTTTGGTCGTCAGAGCCGAATCATCATAACCACCCGAGACAAG
GGCTTGCTCAATAGTTGTGGAGTAAAAACCATCTATGATGTTAAGTGCTTGGACGACAAG
GATTCCCTGCAGATGTTTAAACAGATTGCTTTTGAAGGAGGCAGTCCTCCTTCTGTTGAT
TTTGAACAGCTCTCAATCCGAGCAGCTCGGCTTGCGCACGGGCTTCCTTCTGCCCTTCAA
GCCTATGCCTTATTTCTCCGTGGAAGGGCCAACTCTCCTGAAGAATGGGAAGAAGCAGTA
TGTGGACTTGAAAGTACTCCTGACGAGAATATAATGGAAATCCTGAAAATTAGCTACGAG
GGTTTAGCAAAAGCACATCAGAATGCTTTTCTCCATGTTGCATGTCTCTTTAATGGAGAC
ACTTTCCGGCGTGTCACTTCACTACTTGATGTTAGCAGAATGGAAAGTAACTTGTGGATG
AGAGTTTTAGCAGAG

Last Updated on 06.12.2018 16:11