Sequences from confirmation amplicon


Confirmation sequences for line 230B08 and hit At4g30100 are shown.
Line ID 230B08
Locus At4g30100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14717815
Primers used for PCR 38ra - 8474, show primer details
   
Sequence Name 14-K115950-0022-230-B08C-38ra
GenBank Accession LN492654 [GenBank]
Sequencing primer gene specific primer, 38ra: 5'-GTTCCAGCAGCTTTGCTAATAACT-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K115950-0022-230-B08C-38ra
ATCGCGCAGCACCAGCGCAAGGGGAGGAAGAACTCCAACCTCAGAAGCTTGGGCAGCCTC
GTCGATGACCACCATATCGAAGCCATGAGTAAGACGAGAAAACAGTTTACGGCCACTGCT
TGATACGGTTGTAAAAACAATTTCAGCCTCATTTGCAAAACTTGCCTCAAGACTTGCGCG
AGCTTCTTCAAGATTAAAAGAAGTACCAGCTCGAAACTTTCCCTCAACAATAAGAAGGCG
AGACATTTCAACAAGATCTTTATCTCTGGCTTCAACAACAGCAGAAAGACGCTGTAACAT
TGCATCTCTTGTCTGGTCTCTAACCATAAGAACATCAGGGTCAACGCCAACAGATCCCTG
AGAACGGTTAGCAAACGCTGCAGCAATGAGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 04-K115949-0022-230-B08C-8409
GenBank Accession LN488059 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K115949-0022-230-B08C-8409
CATTTCTCATTGCTGCAGCGTTTGCTAACCGTTCTCAGGGATCTGTTGGCGTTGACCCTG
ATGTTCTTATGGTTAGAGACCAGACAAGAGATGCAATGTTACAGCGTCTTTCTGCTGTTG
TTGAAGCCAGAGATAAAGATCTTGTTGAAATGTCTCGCCTTCTTATTGTTGAGGGAAAGT
TTCGAGCTGGTACTTCTTTTAATCTTGAAGAAGCTCGCGCAAGTCTTGAGGCAAGTTTTG
CAAATGAGGCTGAAATTGTTTTTACAACCGTATCAAGCAGTGGCCGTAAACTGTTTTCTC
GTCTTACTCATGGCTTCGATATGGTGGTCATCGACGAGGCTGCCCAAGCTTCTGAGGTTG
GAGTTCTTCCTCCCCTTGCGCTTGGTGCTGCGCGATGTGTACTTGTCGGTGATCCTCAGC
AGCTTCCTGCTACAGTTATTAGCAAACGGCCTGGAACA

Last Updated on 06.12.2018 16:11