Sequences from confirmation amplicon


Confirmation sequences for line 230E11 and hit At1g51520 are shown.
Line ID 230E11
Locus At1g51520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19107333
Primers used for PCR 07ea - 8474, show primer details
   
Sequence Name 31-K110678-0022-230-E11C-07ea
GenBank Accession LN500210 [GenBank]
Sequencing primer gene specific primer, 07ea: 5'-TTTTCTCTTCGTCTTCTTCACCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K110678-0022-230-E11C-07ea
CTTCTCGGCCTGGATCTGGCGACCAACTTCATAATCTTTTCAAGATCAGAACTTTCGAAC
TTGAGAACGCTTCCAGAGTCATTGACTATTTTCTCTTGCAAGACATGGAACAAAGAGATT
TGATTCGAATTGCTTTTGGTCCTGACTCTCTCATACAGACTTTCTGTCAGAAAGCTAAAG
TCGATTTAGGTTTTTCCTCGAACGGTTTCTCGAGACCCATCAATATCCATGGCCAGTCAT
TGTCCTCGTCTTCTCCCAGAAATGGGTTCTTAGAGTTCTCGAGAAACCCTAGTAACCCTT
TGTCTTCTTCGTTAACTTTGAACACACTCAGAGATAATAAGCCTAACTTCAATTCTAGTC
CTTTTCGTGAAAGTTCATCACTTTTTGCCTCTTCTTCCGGCGATGAGCAGCAGCAACAAC
AATTCAGTAACAACTTTCTGTTCACAAACGACGAGGACCCATTTGCCAATTCTCACAAGA
GGAGTTTCTCTGCTAACGATGCTGGTTTTGAGTCAGAGGAACCATGGTTTGGAGGAGGAA
ATGGTTGTCAATTGATCCATGTAGTATTTCCATGTATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATT
   
Sequence Name 15-K110677-0022-230-E11C-8409
GenBank Accession LN493022 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K110677-0022-230-E11C-8409
TTGAATATCATGGAAATACTACATGGATCAATTGACAACCATTTCCTCCTCCAAACCATG
GTTCCTCTGACTCAAAACAAGCATCGTTAGCAGAGAAACTCCTCTTGTGAGAATTGGCAA
ATGGGTCCTCGTCGTTTGTGAACAGAAAGTTGTTACTGAATTGTTGTTGCTGCTGCTCAT
CGCCGGAAGAAGAGGCAAAAAGTGATGAACTTTCACGAAAAGGACTAGAATTGAAGTTAG
GCTTATTATCTCTGAGTGTGTTCAAAGTTAACGAAGAAGACAAAGGGTTACTAGGGTTTC
TCGAGAACTCTAAGAACCCATTTCTGGGAGAAGACGAGGACAATGACTGGCCATGGATAT
TGATGGGTCTCGAGAAACCGTTCGAGGAAAAACCTAAATCGACTTTAGCTTTCTGACAGA
AAGTCTGTATGAGAGAGTCAGGACCAAAAGCAATTCGAATCAAATCTCTNTGTTCCATGT
CTTGCAAGAGAAAATAGTCAATGACTCTGGAAGCGTTCTCAAGTTCGAAAGTTCTGATCT
TTGAAAAGATTATTGAAGTTGGGTCGCCAGGA

Last Updated on 06.12.2018 16:11