Sequences from confirmation amplicon


Confirmation sequences for line 231G06 and hit At1g12570 are shown.
Line ID 231G06
Locus At1g12570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4278934
Primers used for PCR NH24 - 8474, show primer details
   
Sequence Name 56-K036523-022-231-G06C-NH24
GenBank Accession LN509029 [GenBank]
Sequencing primer gene specific primer, NH24: 5'-AAACAAAAATGTTGGTCCTGTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K036523-022-231-G06C-NH24
CTTATATGGATTGAACCTGATATTTTGCAAAATCAATGTCTACCTTTTTTTCACTATACA
TTTCATGTAAATTCTTGCTTACCACACTTTATTCTAATTCATTTTGTTGCCACTAAATCA
GATTATTAACTAACTATCAAATTACCAATATAATTATTCACGTTTAAGGTACGACCAAGC
CAATAGCCAACGGAGTTGTGTACCGAGACCGGACCGGTCAGGCTCATAGAGCTTACCTAA
AGGAAGGCGCCTTGAGTGAGATCATCCTATCGGCCGGAACCCTAGGGAGCCCACAACTTC
TTATGCTAAGTGGTGTTGGCCCATCGGCTCAATTACAGGCCCAAAACATCACGGTGGTGA
TGGACCAGCCTCATGTGGGTCAAGGCATGTATGACAACCCTATGAATGCCGTGTTCGTTC
CTTCTCCAGTCCCCGTTGAGGTCTCACTCATTGAGGTTGTTGGGATTACCGGGGAAGGAA
CATATGTCGAAGCCGAGTATGATGGATCACCAATTGGCTTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATG
AAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATA
AACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT
   
Sequence Name 32-K036523-022-231-G06C-8409
GenBank Accession LN500493 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K036523-022-231-G06C-8409
GCCATTTAAATTGAAGCCAATTGGTGATCCATCATACTCGGCTTCGACATATGTTCCTTC
CCCGGTAATCCCAACAACCTCAATGAGTGAGACCTCAACGGGGACTGGAGAAGGAACGAA
CACGGCATTCATAGGGTTGTCATACATGCCTTGACCCACATGAGGCTGGTCCATCACCAC
CGTGATGTTTTGGGCCTGTAATTGAGCCGATGGGCCAACACCACTTAGCATAAGAAGTTG
TGGGCTCCCTAGGGTTCCGGCCGATAGGATGATCTCACTCAAGGCGCCTTCCTTTAGGTA
AGCTCTATGAGCCTGACCGGTCCGGTCTCGGTACACAACTCCGTTGGCTATTGGCTTGGT
CGTACCTTAAACGTGAATAATTATATTGGTAATTTGATAGTTAGTTAATAATCTGATTTA
GTGGCAACAAAATGAATTAGAATAAAGTGTGGTAAGCAAGAATTTACATGAAATGTATAG
TGAAAAAAAGGTAGACATTGATTTTGCAAAATATCAGGTTCAATCCATATTAAGATGAAA
AAAATTTAACCATGTGAAAACAGGACCA

Last Updated on 06.12.2018 16:11