Sequences from confirmation amplicon


Confirmation sequences for line 231H01 and hit At1g09970 are shown.
Line ID 231H01
Locus At1g09970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3253072
Primers used for PCR BE11 - 8474, show primer details
   
Sequence Name 07-K028341-022-231-H01C-8409
GenBank Accession LN489129 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K028341-022-231-H01C-8409
TTCGAAGCTCGTCAAATCTCCATCGCCGGTGGAATTTTTCCGGCGATACTGCAATTCGAC
AAGTAAAGCCACGAGAGTTTCTTCAGAGAAACAACCTCCACCGGGAAATCAGCCGTCGCA
TCAAACGGATTATCGCCTAGGCTCAAAACGACGAGACTCGTTGCGTTTCGGAGCGATTTC
CACGGAAAGACGCCGGAAAAAGCGCTGTTGTTGAGATACAGAAACTGTAACTGATTTAAA
GATGAGAACTCAGGGAAAGCACCGGAGAATAAATTGTTGCCGAGATCCAAATACTTCAAA
CTCGTGCAGTTTTTCAAATCGCTGGGGATTATTCCAGATAGTGAATTGAATCCGAGAGAG
AGTTTCTCGAGACTCTGAATCTCACAAACCGAATCGAATGGAAAATTCCCAGATAAACCT
CGACGAGAGAGATCAATCTCTGTACGTTTGCCT
   
Sequence Name 07-K028341-022-231-H01C-BE11
GenBank Accession LN489130 [GenBank]
Sequencing primer gene specific primer, BE11: 5'-AGAGGCAACGTTACAGAGATTGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K028341-022-231-H01C-BE11
AGTCTCGAGAAAACTCTCTCTCGGATTCAATTCACTATCTGGAATAATCCCCAGCGATTT
GAAAAACTGCACGAGTTTGAAGTATTTGGATCTCGGCAACAATTTATTCTCCGGTGCTTT
CCCTGAGTTCTCATCTTTAAATCAGTTACAGTTTCTGTATCTCAACAACAGCGCTTTTTC
CGGCGTCTTTCCGTGGAAATCGCTCCGAAACGCAACGAGTCTCGTCGTTTTGAGCCTAGG
CGATAATCCGTTTGATGCGACGGCTGATTTCCCGGTGGAGGTTGTTTCTCTGAAGAAACT
CTCGTGGCTTTACTTGTCGAATTGCAGTATCGCCGGAAAAATTCCACCGGCGATTGGAGA
TTTGACGGAGCTTCGGAATTTGGAGATTTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGA
TCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTT
CGACGTGTCTACA

Last Updated on 06.12.2018 16:11