Sequences from confirmation amplicon


Confirmation sequences for line 231H10 and hit At1g01570 are shown.
Line ID 231H10
Locus At1g01570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:204964
Primers used for PCR 29by - 8474, show primer details
   
Sequence Name 22-K108417-0022-231-H10C-29by
GenBank Accession LN496145 [GenBank]
Sequencing primer gene specific primer, 29by: 5'-GTTGTCGTTTTGGTTAATGTGTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K108417-0022-231-H10C-29by
TACGCTTTGGTTTACCAGAGCTTCACGTAGTCTTTACGGTGTTTCCAGAATTTCTCAGAG
GCGGCGATTCCGAAAACGACGTGCTTGAGTTCGGTTTGGTCCGGTTCATGAACCGACCCG
GATCCGGATACGACGGGTGAAACAGAGAATTTGATCAGAGGTTGAGAAGATTACGAGATG
AGATTTAAAGGGT
   
Sequence Name 06-K108416-0022-231-H10C-8409
GenBank Accession LN488847 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K108416-0022-231-H10C-8409
CATTTACAATTGAATCTCTTCTCTGAAACCCTCCCCCAGTGACTCACCACCACGTCAAAA
AAATAAAAAAACGGCGTAGTTTCGGTTACTTCGTCGTCGTCTTCCTCATTTCGCCGGAAG
AATTATCCGCCATGGGAGACGATAAGAAAGATCAGAAGCAGCTTTCTTCATACTTGAAAG
CTAAAAACTTTTCATGTGATTCACATTTTTCATTTTATTGGCTAATGTCGAGGCTAATCT
TCTTAATTCTCGCCATTCTCTTCTCTCTTCAATTCGTTTTCTACCCTTTAAATTTCATCT
CCTCTTCTTCTCAACCTCTGATCAAACTTCTCTGTTTCACCCGTCGTATCCGGATCCGGG
TCGGTTAATGAACCGGACCC

Last Updated on 06.12.2018 16:11