Genotyping and primer details


Genotyping and primer details for 231H10 - At1g01570
Line ID 231H10
Locus At1g01570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:204964 of line 231H10 Chr1:204964
   
Insertion allele amplicon 8474 - 29by
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 29by: 5'-GTTGTCGTTTTGGTTAATGTGTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:205478
   
wt amplicon (over InsPos) 21da - 29by
wt amplicon start Chr1:204419
wt amplicon end Chr1:205478
wt amplicon size 1060 bp
Locus specific primer 29by: 5'-GTTGTCGTTTTGGTTAATGTGTTG-3'
2nd locus specific primer 21da: 5'-ACACTAGTCTTATCTGATTGGCCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11