Sequences from confirmation amplicon


Confirmation sequences for line 232F05 and hit At3g07690 are shown.
Line ID 232F05
Locus At3g07690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2458289
Primers used for PCR RZ13 - 8474, show primer details
   
Sequence Name 33-K017464-022-232-F05C-RZ13
GenBank Accession LN500794 [GenBank]
Sequencing primer gene specific primer, RZ13: 5'-TGCTGATATTGTCATCAATGGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K017464-022-232-F05C-RZ13
GTTTCAGGTGTTCACTGAGATTAGTAAGTATTGGAAAGAGAGGGTTAATGCTCCTGTTAT
TATCTCTTTGGCTAAAGGTGTGGAAGCAGAGTTTGAGCCTCATCCTCGGATTGTTACTCC
TACGCAAATGATCCATCGAGCGAGTAAATTCACTTCCCATTGTCTTTTATCTGATTGCTA
GATGAATATTTGATATCATCTTCCTTAGTATTTTTGTTGATGTAGAGAAACTTTCTATTT
TTATTAAGGTTATATGAGTATTGACTGTGTTGGCGTTGAAGTGTATGATCAGTAGTTGTT
GTCATTGGAAATTCTACAGTTTGCTTGTCATATTCTCTTTGCAGCTGGTATTCCTCTTGA
GAACATTCTCTACCTTGGGGGACCTAATATTGCTTCCGAGGTATACAATAAAGAGTACGC
CAATGCACGGATTTGCGGGTCTGAAAAATGGAGGAAGCCTTTGGGAAAGTTTCTAAGGCA
GTCTCATTTCATAGTCTGGGATAATAGTGACCTCATTACTCATGAAGTAATGGGTGGCTT
GAAGAATGTTTATGCCATTGGTCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAANATGAAAGTA
CATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAAGC
   
Sequence Name 34-K017464-022-232-F05C-8409
GenBank Accession LN501219 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K017464-022-232-F05C-8409
ATGAAGCCACTTACAATTGAATATATCCTGGACCAATGGCATAAACATTCTTCAAGCCAC
CCATTACTTCATGAGTAATGAGGTCACTATTATCCCAGACTATGAAATGAGACTGCCTTA
GAAACTTTCCCAAAGGCTTCCTCCATTTTTCAGACCCGCAAATCCGTGCATTGGCGTACT
CTTTATTGTATACCTCGGAAGCAATATTAGGTCCCCCAAGGTAGAGAATGTTCTCAAGAG
GAATACCAGCTGCAAAGAGAATATGACAAGCAAACTGTAGAATTTCCAATGACAACAACT
ACTGATCATACACTTCAACGCCAACACAGTCAATACTCATATAACCTTAATAAAAATAGA
AAGTTTCTCTACATCAACAAAAATACTAAGGAAGATGATATCAAATATTCATCTAGCAAT
CAGATAAAAGACAATGGGAAGTGAATTTACTCGCTCGATGGATCATTTGCGTAGGAGTAA
CAATCCGAGGATGAGGCTCAAACTCTGCTTCCACACCTTTAGCCAAAGAGATAATAACAG
GAGCATTAACCCTCTCTTTCCAATACTTACTAATCTCATTGAACACCTGAAACGTCTCAG
TTGATGGTAAC

Last Updated on 06.12.2018 16:11