Genotyping and primer details


Genotyping and primer details for 232F05 - At3g07690
Line ID 232F05
Locus At3g07690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2458289 of line 232F05 Chr3:2458289
   
Insertion allele amplicon 8474 - RZ13
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer RZ13: 5'-TGCTGATATTGTCATCAATGGTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2457684
   
wt amplicon (over InsPos) 01ep - RZ13
wt amplicon start Chr3:2457684
wt amplicon end Chr3:2458614
wt amplicon size 931 bp
Locus specific primer RZ13: 5'-TGCTGATATTGTCATCAATGGTTT-3'
2nd locus specific primer 01ep: 5'-AAGTTCTCCTTTTGCAAGTTTCTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11