Sequences from confirmation amplicon


Confirmation sequences for line 232G05 and hit At1g56385 are shown.
Line ID 232G05
Locus At1g56385 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:21106788
Primers used for PCR 45oh - 8474, show primer details
   
Sequence Name 77-K114716-0022-232-G05C-45oh
GenBank Accession LN513587 [GenBank]
Sequencing primer gene specific primer, 45oh: 5'-GCTCTAAATTTGAAGTGGGTGGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K114716-0022-232-G05C-45oh
CACCCGCGCACATATTCATTTAACCATTGCATAATAATGAACATGAGTACAGATGGTAAA
TTTGATTATTATCACCATATAAACAACAAAACATGTACAATATTTTTTTTATTAATCCCA
GTTGTAACAAATATCATATTTTTTAGTCAGTCCATTAAGAGCACAAGCTCCATACGGTCT
GATGGACCAAAAACAGTGTCTGCACTGACCTTTGTCACTATTATCTCTGCTACCTTTATA
TATAGTAAAGCTGCGTCGTTTATTACCAAAATGAAACATACAACTATAGAGATCTCCTCC
CCAGAAACTTTTCGTGAATTGAGTGAATTTTGTCATACGCCCCGGTAAAAATTCTCGGTC
ACGAGCTTGCCCCTTTATGTTATTATAGCAACTAGCAGTTAGCACTAAACCACGTCCGAT
ATCGTTTCTGAGGTAAACATGTATTGTCGGCCATATCTCCCATCCAAATATATGGGTATT
CTTTATTCTAAATACACCGTTTTGAGATAATGCTACTTTCATGAGCAACGATNGTATGAG
AAGCATTATCAGTTTTCTATGGGAAACCATCTTTTATTCTTTATATTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGATATCCG
   
Sequence Name 29-K114715-0022-232-G05C-8409
GenBank Accession LN499404 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K114715-0022-232-G05C-8409
TTACATTGAATATAAGAATAAAAGATGGTTTCCCATAGAAAACTGATAATGCTTCTCATA
CTATCGTTGCTCATGAAAGTAGCATTATCTCAAAACGGTGTATTTAGAATAAAGAATACC
AATATATTCGGATGGGAGATATGGCCGACAATACATGTTTACCTCAGAAACGATATCGGA
CGTGGTTTAGTGCTAACTGCTAGTTGCTATAATAACATAAAGGGGCAAGCTCGTGACCGA
GAATTTTTACCGGGGCGTATGACAAAATTCACTCAATTCACGAAAAGTTTCTGGGGAGGA
GATCTCTATAGTTGTATGTTTCATTTTGGTAATAAACGACGCAGCTTTACTATATATAAA
GGTAGCAGAGATAATAGTGACAAAGGTCAGTGCAGACACTGTTTTTGGTCCATCAGACCG
TATGGAGCTTGTGCTCTTAATGGACTGACTAAAAAATATGATATTTGTTACAACTGGGAT
TAATAAAAAAAATATTGTACATGTTTTGTTGTTTATATGGTGATAATAATCAAATTTACC
ATCTGTACTCATGTTCATTATTATGCAATGGTTAAATGAATATGTGCGCGGGTGTGGGCT
AAGAATTTTTACTAATACCACCCACTTCAATTTTTAGA

Last Updated on 06.12.2018 16:11