Sequences from confirmation amplicon


Confirmation sequences for line 232G05 and hit At5g19620 are shown.
Line ID 232G05
Locus At5g19620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6624425
Primers used for PCR DL13 - 8474, show primer details
   
Sequence Name 55-K017403-022-232-G05C-DL13
GenBank Accession LN508652 [GenBank]
Sequencing primer gene specific primer, DL13: 5'-AGTAAGAACGAAAGATGGTGAGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K017403-022-232-G05C-DL13
TTTGAAAGCTTGTAGAGCTAATTCAGCGTTGACTATTCGTGAGGTACAGGAGGATGTACA
TAGGATTATAGAAAGTGGGTACTTTTGCTCTTGTACTCCTGTTGCTGTTGATACACGAGA
CGGGATTCGGTTGATGTTTCAGGTAATATTTTGATTGTTACTCTTTTTGTTGGAGGAGAA
AGGTCTCGTTTTTATGTTTCTGGGATGCAAATAATTCTCATTTTCTTGCCATAAGGTAGA
ACCAAACCAAGAATTTCGTGGGTTAGTCTGTGAAAATGCAAATGTTCTTCCTTCCAAGTT
CATACATGAAGCTTTCCGAGATGGGTTTGGTACGTATCCTCCAATTTAGCTTGTTTTTTT
TTCTTCTGTACTGTACTAGACTTATCTGTCAATTCATTGATTTACGTTGGGAGTAAAATG
GATTAATGATATTGGAACCTGACAAATATTTCGAGTACTAATGATGAAGCTAAGAATTTT
CTAACACATGGTGTAAAATGTGTTAGCCTTGAATGAAGAAGATAGATTAGAGGTAGGACA
TTTACATTACCAATACATATTCATGTAATTAACCATTTGTTATTTCCAGGATATATTCAA
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
   
Sequence Name 56-K017403-022-232-G05C-8409
GenBank Accession LN508964 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K017403-022-232-G05C-8409
ACATGAAGCCATTTACAATTGAATATATCCTGGAAATAACAAATGGTTAATTACATGAAT
ATGTATTGGTAATGTAAATGTCCTACCTCTAATCTATCTTCTTCATTCAAGGCTAACACA
TTTTACACCATGTGTTAGAAAATTCTTAGCTTCATCATTAGTACTCGAAATATTTGTCAG
GTTCCAATATCATTAATCCATTTTACTCCCAACGTAAATCAATGAATTGACAGATAAGTC
TAGTACAGTACAGAAGAAAAAAAAACAAGCTAAATTGGAGGATACGTACCAAACCCATCT
CGGAAAGCTTCATGTATGAACTTGGAAGGAAGAACATTTGCATTTTCACAGACTAACCCA
CGAAATTCTTGGTTTGGTTCTACCTTATGGCAAGAAAATGAGAATTATTTGCATCCCAGA
AACATAAAAACGAGACCTTTCTCCTCCAACAAAAAGAGTAACAATCAAAATATTACCTGA
AACATCAACCGAATCCCGTCTCGTGTATCAACAGCAACAGGAGTACAAGAGCAAAAGTAC
CCACTTTCTATAATCCTATGTACATCCTCCTGTACCTCACGAATAGTCAACGCTGAATTA
GCTCTACAAGCTTTCAAAGCAGCCAATGCCTCCATTTCCAAATCCTTCCTCTCAAGTTCC
TCACCAT

Last Updated on 06.12.2018 16:11