Sequences from confirmation amplicon


Confirmation sequences for line 233G02 and hit At2g18150 are shown.
Line ID 233G02
Locus At2g18150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7893180
Primers used for PCR 03vy - 8474, show primer details
   
Sequence Name 21-K118356-0022-233-G02C-03vy
GenBank Accession LN495861 [GenBank]
Sequencing primer gene specific primer, 03vy: 5'-TTACCAACTCTATTGCCTTGCTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K118356-0022-233-G02C-03vy
ATACTTTCGATTCTTCTTCTAATCCTTCATATCTCCTACCATCACAAAGTCATCTCCTTC
TTCTTTCTTGATCTAGCTAGCGGAAAATTTGACAATGGCAAGAATCGGAAGCTTTCTCAT
TATTCTTTACCTCATTTACGCTCTTACTCTCTGCATCTGCGACGACGACGAGAGTAACTA
TGGCGGCGACAAAGGGAACCTCTTCCCAGGTTTCTACAGAAGCTCGTGCCCTAGAGCCGA
GGAGATCGTGAGGTCAGTTGTAGCCAAAGCTGTTGCAAGGGAGACTCGTATGGCTGCTTC
TCTCATGAGGCTCCATTTCCACGATTGTTTCGTTCAGGTACTAGAGAGGTTTTTTTTAAG
GAACTTGCTCAATAAGTTTAGTGTTGAGACACATCATTAATTAATTTTATCTGTTCGAAT
GAAAATGTGTAGGGTTGTGATGGATCGTTGCTTCTAGACACCAGTGGGAGTATAGTTACT
GAGAAGAACTCTAACCCGAACAGCAATCTACATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCG
   
Sequence Name 21-K118355-0022-233-G02C-8409
GenBank Accession LN495860 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K118355-0022-233-G02C-8409
GTTCGGGTAGAGTTCTTCTCAGTAACTATACTCCCACTGGNTGTCTAGATGCAACGATCC
ATCACAACCCTACACATTTTCATTCGAACAGATAAAATTAATTAATGATGTGTCTCAACA
CTAAACTTATTGAGCAAGTTCCTTAAAAAAAACCTCTCTAGTACCTGAACGAAACAATCG
TGGAAATGGAGCCTCATGAGAGAAGCAGCCATACGAGTCTCCCTTGCAACAGCTTTGGCT
ACAACTGACCTCACGATCTCCTCGGCTCTAGGGCACGAGCTTCTGTAGAAACCTGGGAAG
AGGTTCCCTTTGTCGCCGCCATAGTTACTCTCGTCGTCGTCGCAGATGCAGAGAGTAAGA
GCGTAAATGAGGTAAAGAATAATGAGAAAGCTTCCGATTCTTGCCATTGTCAAATTTTCC
GCTAGCTAGATCAAGAAAGAAGAAGGAGATGACTTTGTGATGGTAGGAGATATGAAGGAT
TAGAAGAAGAATCGAAAGTATATATAGGCCAGAGGGTTCGTGGATTATAGCGTCATGAAG
AGGAAAAAGCAAGGCAA

Last Updated on 06.12.2018 16:11