Sequences from confirmation amplicon


Confirmation sequences for line 233H09 and hit At1g53040 are shown.
Line ID 233H09
Locus At1g53040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19764938
Primers used for PCR 45vr - 8474, show primer details
   
Sequence Name 50-K117939-0022-233-H09C-45vr
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 45vr: 5'-TTGGTTCTTTTGACAGAAACTCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K117939-0022-233-H09C-45vr
CAATTTTTTCTCCCCCGGGGAAAATTTTCCGGGGACCCCTTTTCCCCGGTTTACCCGGAT
TCTTTTTTCCCGGGGTGGTTTTTCCCCCGGGCAATTTTCCCGGTGGTAAAACCCGGGGCC
CGGGAAAAACTTCTAAAAAACTCCGGGGGGGTTTTCGGGGCCCCAAAACTTTCTTTTTAT
TTCCCGTTCCTGCGGGGAAAACCGGTTCAAGGTTTTCCCGATTCAAACCCCAACCGAAAA
GGGATTTTCCCCCGCTTTTCCCGTTTTCCAAAATTTGGGTTTTTTCCCGCCCGAAAAAAA
TTGGGGTCCCCAAACAATTTTTCCCCCTTTTTTTCTCCCGGAAATTTTTATGGGCCTCCT
TTCCGGGAAACCCCCCGGGAACACCCAGGAAAAAGGTTTTTTTTTTTGGGGAGGGGGAAG
ATTTTTTCCCTTTTTTTTTTAAATTCAAAGGGGGTCTTTTCCAACAAAAAAAAAAAAA
   
Sequence Name 41-K117938-0022-233-H09C-8409
GenBank Accession LN504245 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K117938-0022-233-H09C-8409
GAGACAAATAAGAGAGAAAGTTGATTGGAGCATCAATATGTCTCGTTGGACTGTGAAAGA
CGCAACTTTGTGAAACAGGTAAAAGCAGAGGAAACTTCGGTTTTGTTTCGGTTTTGATAT
GGGAAAACATGATACTTGTTTATCCTTGATGACGGGTATATCATAGAGACTTTTTGCTGA
CGATGAAACCTCCTCGAGCTTCTTCTATAGTTCTTCCCGAGCCGCTGGTTTTACCACATG
GAAGATTAGCCGGAGGTATAGCGGCGCCGGGTAAGAAGACTCCTGGACAACGCGGGAAAA
GGCGTCACCGGAAAGTTTCTTCCGGTGGCAAAACCAAGTGAAAGAGAGAGCCCCTCCGCT
CTTTTTCTTCTGCAAAATTTGTTTTTTATGC
Primers used for PCR 38vw - 8474, show primer details
   
Sequence Name 38-K118262-0022-233-H09C-38vw
GenBank Accession LN503145 [GenBank]
Sequencing primer gene specific primer, 38vw: 5'-TACAGACGATAACAAGAGAGTGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K118262-0022-233-H09C-38vw
GGTTAAAAGAATTGAGAAATAAATAGCCAAGATTTGAACGTTTCCTGATTTAACAAATAC
GAATTGGGTAAAACGTTTATGCATTTTTACAGGTTCCGAAGCTTTTATTGCATAGATTAT
TCCCAAATGTCCGGTACTCTATATGGGTTGATGCAAAGCTCCAGCTTGTTGTAGATCCAT
ATCAAATCCTTGAAAGGTAGAGAAATTGACTCCTCTGTCTCTCTCTTTCAACATATTTTG
TTCCAAGCAGTTAGCTTTATTTGTCTTCTCAATGTTCAGGTTCTTGTGGAGAACGAATTC
TAGTTTTGCAATCTCGAGACATTACCGACGTTTTGATGTGTTTGTAGAGGCTGAAGCGAA
TAAGGCCGCAAGAAAGTACGACAATGCCTCAATAGATTACCAAGTAGAGTTCTACAAGAA
GGAAGGTTTAACACCTTATACGGAGGCTAAGCTTCCAATAACAAGTGGTAATGGTTTTAA
CCATATGAGGATGATAAAGTTCCACAATTTTCTTCTGTTCAATGATCACAATTGAAACAT
TTCAAATCGTTTGGCAGATGTCCCTGAAGGTTGTACCATCATAAGAGAACACATACCGAT
CACAAACCTTTTCACATGTGTATGGTTCAACGAGGTAGATCGGTTTACTTCAAGAGATCA
GCTAAGCAATTGGTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTNCATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAATGT
   
Sequence Name 18-K118261-0022-233-H09C-8409
GenBank Accession LN494505 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K118261-0022-233-H09C-8409
AGTAAACCGATCTACCTTCGTGAACCATACACATGTGAAAAGGTTTGTGATCGGTATGTG
TTCTCTTATGATGGTACAACCTTCAGGGACATCTGCCAAACGATTTGAAATGTTTCAATT
GTGATCATTGAACAGAAGAAAATTGTGGAACTTTATCATCCTCATATGGTTAAAACCATT
ACCACTTGTTATTGGAAGCTTAGCCTCCGTATAAGGTGTTAAACCTTCCTTCTTGTAGAA
CTCTACTTGGTAATCTATTGAGGCATTGTCGTACTTTCTTGCGGCCTTATTCGCTTCAGC
CTCTACAAACACATCAAAACGTCGGTAATGTCTCGAGATTGCAAAACTAGAATTCGTTCT
CCACAAGAACCTGAACATTGAGAAGACAAATAAAGCTAACTGCTTGGAACAAAATATGTT
GAAAGAGAGAGACAGAGGAGTCAATTTCTCTACCTTTCAAGGATTTGATATGGATCTACA
ACAAGCTGGAGCTTTGCATCAACCCATATAGAGTACCGGACATTTGGGAATAATCTATGC
AATAAAAGCTTCGGAACCTGTAAAAATGCATAAACGTTTTACCCAAATCGTATTTGTTAA
ATCAGGAAACATTCAAATCTTGGCTATNTATTCTCAATTCTNTTAACCTTTCCATTACGC
CGTGCATCGGTATAAGGGACATTATGTACAACAATGATCCTC

Last Updated on 06.12.2018 16:11