Sequences from confirmation amplicon


Confirmation sequences for line 234E05 and hit At5g07670 are shown.
Line ID 234E05
Locus At5g07670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2430502
Primers used for PCR BK10 - 8474, show primer details
   
Sequence Name 06-K104736-0022-234-E05C-BK10
GenBank Accession LN488799 [GenBank]
Sequencing primer gene specific primer, BK10: 5'-AGTTTCCAAAACGAAAGCTAACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K104736-0022-234-E05C-BK10
AAATATCATTTTTTATAACAAAATCTAAAATCCCTAAACACAAAATTTTTGCTCTGTGAA
TGTAAAAGTTCTCTAGTTCCTCAAAGCTTCAAACTTTCCATTTCTATTGCAGAAATCCGT
TAAAGCTCTTTCTCGGGAAAATCAAAACCGAGAAAAAACCTTCAAGAAACGTTGACGGAG
AATGTGAAGTATCATGTCTTATAATACGGGGAAGGAGAGTAGTCCAGTTTCGCCATTGAA
GAAGCGACGAGCAAGCTGGTCGGAACTTTGGGTTATCCTATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 22-K104737-0022-234-E05C-8409
GenBank Accession LN496100 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K104737-0022-234-E05C-8409
TTACATTGAATAAGGATAACCCAAAGTTCCGACCAGCTTGCCCGTTAATTCTTCAATGGC
GAAACTGGACTACTCTCCTTCCCCGTATTATAAGACATGATACTTCACATTCTCCGTCAA
CGTTTCTTGAAGGTTTTTTCTCGGTTTTGATTTTCCCGAGAAAGAGCTTTAACGGATTTC
TGCAATAGAAATGGAAAGTTTGAAGCTTTGAGGAACTAGAGAACTTTTACATTCACAGAG
CAAAAATTTTGTTTTTAGGGATTTTAGATTTTGTTTATAAAAAATGATATTTTTGTTGTC
TCTCTCTAAAGACGAAAGATTCGGTCTCTTTTTGTTAGCTTTCGTTTTGGAAAC

Last Updated on 06.12.2018 16:11