Sequences from confirmation amplicon


Confirmation sequences for line 234F11 and hit At5g28380 are shown.
Line ID 234F11
Locus At5g28380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:10339601
Primers used for PCR 42xp - 8474, show primer details
   
Sequence Name 79-K119579-0022-234-F11C-42xp
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 42xp: 5'-ACGACAATTCTGCGTTCTATCCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K119579-0022-234-F11C-42xp
AACAACACTTTTATCTTGGGACTATTGTTTTCAGAATTGAGTGCAAGTGTGGTTAAGTGC
AAGTGTGGTTCATGTGGGTCAAGGAAGCAGTCACCAAGTGAATGGGAAAGGCATACAGGC
TGCAGAGCCAAAAAGTGGAAGTTTAGTGTAAGAGTGAAAGACACAATGCTTACCTCTATA
AAAATGGGTACATCCGTTATGTGTTGGAATATTGAGAGAAAAGCAAAAACAGAGTAAGAG
ACATTTGTCTCAGAGACATTTGTCTCTTTTCTTGTAAAGAAGACAAGAACAAAACTTTGT
TTTATTAAAAATGAAACTTGTTGTTTTACTCGACTAGATACACACACTTATGTACAGGTC
TTAGGTACATAACCGACCAAACTAAACATTGTGACTGTTGAAACTAAGATAAACTGCCCA
AACTAAAGTTACGACTCTTACAACCACCTTTTGCTATAACATAGAAACTTAATAATCAAA
TAACATATTACCAAACTCAATTATAAACTAACATTATGCTTAACTCTCTCATAAACAGGC
CCTAATATATACCCCTGAATTTTCCATGCAAATAAGATATTACAGATTGTGTGCGTCCGA
AATCCAAACACAAGCCGAACAGAGTGGTGTATTTGATTCTCAATCTGCAGCAAGATGATG
GCTTATATACAGTAGATCAAACACAAAGTGTATGCTTCTAGGGGTCTTGTTTATTATTGG
ACATTGGAGAGTCGTTCATTCTTAAGTATTCCCTGGCAAAGGTACTAATATTTCATCTCT
GTTACTGCTGATGTTTCAGAAGATTGATCTGGAGGCGATATAATACGACAGACTTATTAA
GGGACCAACCAAAGCATCATTCGTTGGGTACGACGAAGCCATCTCTCTGTTCGATTACTT
TTTCTCGTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
   
Sequence Name 55-K119578-0022-234-F11C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K119578-0022-234-F11C-8409
TCGGAAGNAGCCATTTACATTGAACGAGAAAAGTAATCGAACAGAGAGATGGCTTCGTCG
TACCCAACGAATGATTGCTTGGTTGGTCCCTTAATAAGTCTGTCGTATTATATCGCCTCC
AGATCAATCTTCTGAAACATCAGCAGTAACAGAGATGAAATATTAGTACCTTTGCCAGGG
AATACTTAAGAATGAACGACTCTCCAATGTCCAATAATAAACAAGACCCCTAGAAGCATA
CACTTTGTGTTTGATCTACTGTATATAAGCCATCATCTTGCTGCAGATTGAGAATCAAAT
ACACCACTCTGTTCGGCTTGTGTTTGGATTTCGGACGCACACAATCTGTAATATCTTATT
TGCATGGAAAATTCAGGGGTATATATTAGGGCCTGTTTATGAGAGAGTTAAGCATAATGT
TAGTTTATAATTGAGTTTGGTAATATGTTATTTGATTATTAAGTTTCTATGTTATAGCAA
AAGGTGGTTGTAAGAGTCGTAACTTTAGTTTGGGCAGTTTATCTTAGTTTCAACAGTCAC
AATGTTTAGTTTGGTCGGTTATGTACCTAAGACCTGTACATAAGTGTGTGTATCTAGTCG
AGTAAAACAACAAGTTTCATTTTTAATAAAACAAAGTTTTGTTCTTGTCTTCTTTACAAG
AAAAGAGACAAATGTCTCTGAGACAAATGTCTCTTACTCTGTTTTTGCTTTTCTCTCAAT
ATTCCAACACATAACGGATGTACCCATTTTTATAGAGGTAAGCATTGTGTCTTTCACTCT
TACACTAAACTTCCACTTTTTGGCTCTGCAGCCTGTATGCCTTTCCCATTCACTTGGTGA
CTGCTTCCTTGACCCACATGAACCACACTTGCACTTAACCACACTTGCACTCAATTC

Last Updated on 06.12.2018 16:11