Sequences from confirmation amplicon


Confirmation sequences for line 234H08 and hit At1g50840 are shown.
Line ID 234H08
Locus At1g50840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18843790
Primers used for PCR 04jg - 8474, show primer details
   
Sequence Name 27-K113214-0022-234-H08C-8409
GenBank Accession LN498470 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K113214-0022-234-H08C-8409
CATTTACAATTGAATATATCCTGCAAATCACCAGATCAACATGTTCAATTGAATGTAAGT
GACTGATCCATATTGACGCCGGAAATCTACGAGACCTTCCTAGTAAAGTGAGTACATACC
CATCTTCTATAGCTTCTTTCTTACGCATTTCTTGCCATTTCCTTACTTCTTGCCTGTCAT
TGTACCAGAGATCGACTGTTTCCTGAGCTTCTTTAGTGGAGACCTTTAGATGATAAAAAA
GTTTGAACAGTTTTAGACTCATCACATCAACGCATGTTTATATCAACCTAATAGTTTCAA
TAGTATTTTTCCACTACCTTCCAATCTCTAGAAAGCCCGACCGCGGTTTTCCCATACGCA
ATTGAAAAGTTAAGC
   
Sequence Name 27-K113215-0022-234-H08C-04jg
GenBank Accession LN498471 [GenBank]
Sequencing primer gene specific primer, 04jg: 5'-TGCTTAACTTTTCAATTGCGTATG-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K113215-0022-234-H08C-04jg
GGAAAAACTATTGAAACTATTAGGTTGATATAAACATGCGTTGATGTGATGAGTCTAAAA
CTGTTCAAACTTTTTTATCATCTAAAGGTCTCCACTAAAGAAGCTCAGGAAACAGTCGAT
CTCTGGTACAATGACAGGCAAGAAGTAAGGAAATGGCAAGAAATGCGTAAGAAAGAAGCT
ATAGAAGATGGGTATGTACTCACTTTACTAGGAAGGTCTCGTAGATTTCCGGCGTCAATA
TGGATCAGTCACTTACATTCAATTGAACATGTTGATCTGGTGATTTGCAGGATATATTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CACGGTTATT

Last Updated on 06.12.2018 16:11