Sequences from confirmation amplicon


Confirmation sequences for line 235H03 and hit At1g73830 are shown.
Line ID 235H03
Locus At1g73830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27760755
Primers used for PCR MJ19 - 8474, show primer details
   
Sequence Name 28-K023359-022-235-H03C-8409
GenBank Accession LN498672 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K023359-022-235-H03C-8409
TAGATTTCCCGGACATGAAGCCATGTAGATATAGTCCCCGGATGTTATAAGGTGATTATA
TTTTGCTTAGACTTTAAGAAAATATATTATTAGTTCTTTTATTGAATATATATTTGGTGA
TGCAGACAATGGGAATGGCTACTATGCTTGATGAGATAATTAATTACGTCCAGTCCTTAC
AAAATCAAGTCGAGGTATAACAATAACAATTAATTTCTCTTAAGTTATAGGTCATCTAAC
CAATTCCATTCGTTTCAATAATTATACTAATTTACTTTCTTTTTTATGTGAACTCAGTTT
TTATCTATGAAGCTTACAGCAGCAAGTTCG
   
Sequence Name 29-K023359-022-235-H03C-MJ19
GenBank Accession LN499115 [GenBank]
Sequencing primer gene specific primer, MJ19: 5'-AGTCATAATACGAACTTGCTGCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K023359-022-235-H03C-MJ19
CTTCATAGATAAAAACTGAGTTCACATAAAAAAGAAAGTAAATTAGTATAATTATTGAAA
CGAATGGAATTGGTTAGATGACCTATAACTTAAGAGAAATTAATTGTTATTGTTATACCT
CGACTTGATTTTGTAAGGACTGGACGTAATTAATTATCTCATCAAGCATAGTAGCCATTC
CCATTGTCTGCATCACCAAATATATATTCAATAAAAGAACTAATAATATATTTTCTTAAA
GTCTAAGCAAAATATAATCACCTTATAACATCCGGGGACTATATCTACATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGA
AAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAA
ACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11