Genotyping and primer details


Genotyping and primer details for 235H03 - At1g73830
Line ID 235H03
Locus At1g73830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:27760755 of line 235H03 Chr1:27760755
   
Insertion allele amplicon 8474 - MJ19
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MJ19: 5'-AGTCATAATACGAACTTGCTGCTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:27761068
   
wt amplicon (over InsPos) 31dy - MJ19
wt amplicon start Chr1:27760263
wt amplicon end Chr1:27761068
wt amplicon size 806 bp
Locus specific primer MJ19: 5'-AGTCATAATACGAACTTGCTGCTG-3'
2nd locus specific primer 31dy: 5'-TTCTTCACAATTCGATACCAAGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11