Sequences from confirmation amplicon


Confirmation sequences for line 236D09 and hit At5g06420 are shown.
Line ID 236D09
Locus At5g06420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1962777
Primers used for PCR 15uw - 8474, show primer details
   
Sequence Name 72-K117671-0022-236-D09C-15uw
GenBank Accession LN512793 [GenBank]
Sequencing primer gene specific primer, 15uw: 5'-TAGTTTCCGTCTCTGTTTGGAGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K117671-0022-236-D09C-15uw
TTGAAGATTTTTGGATTCATACTGAGAAGACCCAAACTATGTTGCAAGCTCTACTTACCA
GAATGTTTAAGAATATTGAGTTTGACGACTTAAATTCTGACGATGACTTACTAGGAAGTT
GACTCCAAAACTTTTTCAGTTTGTTTAAATTTAAAAGTTAATCAGTTTGAAACAACCATC
GTCATCCGAATATACAGTATATAGGCCAGTGAGTACAACAAAACAAAAAGCTTTCCTCAA
GCAAATAATCAAATGTATAGTTTTTGCCTTTTTGTCCTGTGAAAGAAGGACTTCACAATC
CTTGTTCAGCTTTACTCCGTTCTTCAGCCATTCTCTTCTTGATCTCATGTGCTGCATTGA
AAATCCCCATTGTTGGTTGGTTACACTACATGGCTTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTTATTA
   
Sequence Name 24-K117670-0022-236-D09C-8409
GenBank Accession LN497166 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K117670-0022-236-D09C-8409
GCCATTTACATTGAAGCCAGTTAGTGTAACCAACCAACAATGAGGGATTTTCAATGCAGC
ACATGAGATCAAGAAGAGAATGGCTGAAGAACGGAGTAAAGCTGAACAAGGATTGTGAAG
TCCTTCTTTCACAGGACAAAAAGGCAAAAACTATACATTTGATTATTTGCTTGAGGAAAG
CTTTTTGTTTTGTTGTACTCACTGGCCTATATACTGTATATTCGGATGACAATGGTTGTT
TCAAACTGATCAACCTTTTAAATTTAAGCAAACTGAATGAGTTTTGGACTCGACTTCCAA
GGACGTCATCGTCAGAGTTTAACTCTTCAAACTCAATATTCCTAAACATTCTGGTAAGTA
GAGGTTGCAACAAAGTTCGGGTCTGCTCATTGTGAACCCAACAAGCTTTCAAATGACTTG
TGTAACAAGAACAGTAAAACATTCTGCACAATCTCCGAGCTATAACGGTAAACTAAGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC

Last Updated on 06.12.2018 16:11