Sequences from confirmation amplicon


Confirmation sequences for line 236D11 and hit At1g79510 are shown.
Line ID 236D11
Locus At1g79510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29909101
Primers used for PCR BW20 - 8474, show primer details
   
Sequence Name 03-K104337-0022-236-D11C-BW20
GenBank Accession LN487471 [GenBank]
Sequencing primer gene specific primer, BW20: 5'-GGTGACCATATCCAAAACAGAAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K104337-0022-236-D11C-BW20
AGGCCAGTTATCGACCTTATGCTCATAGATTTTGCCATTTCGGTCCAGTTTATAGCGAGA
AGTACCCTGAAACTCTCCCTTTGCCTCCCATGGGACTCTAGGTACACCTTTAAGATTCCA
CCTGATGAGAATCATGTTCTCTGATGGTTGCCAAACCCTGAAGATCTCAAGTGAGATATC
CCTGAAGAGAATCTTGCCATGAAATCTAAGTGCCCAGAAGATCAATTTGTAGTTGTCAAT
CCCAGAGAACGTGTTCATTGGATCCACTAATGTTATATCATCCCTGAAAGAGGAAACACA
AATGCTTTCATTGACAGGTTTAAGGAAGGAATCACCTAAAGGAGAAAGAGGAAAACACAC
CTGTAAATGTCGTAATTGAGATCTTTGGTGAAGAGGAGAGGCAAATCTTCGCGAAGGGTA
CGTACAGCTAAACCGAGATTGATGTAGAAGTCATCTTTGCTGCCGTGCTGGTACTTATCC
TGCGCACTTGGTTTGGTCTTAACCTGCGCACCTCGAACAGGAGGAGGAGCCTGGACGCTA
AAGAGAGAAAGTGGAGAGAGACTATCTTCTTCAAACTCGTAGCTATTGGACGATGATGAT
GATGATGATAAAGCTTGGTTGGAATAAACTCGAATATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTTCATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 19-K104338-0022-236-D11C-8409
GenBank Accession LN494743 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K104338-0022-236-D11C-8409
GATATTCGAGTTTATTCCACCAAGCTTTATCATCCTCATCATCATCGTCCAATAGCTACG
AGTTTGAAGAAGATAGTCTCTCTCCACTTTCTCTCTTTAGCGTCCAGGCTCCTCCTCCTG
TTCGAGGTGCGCAGGTTAAGACCAAACCAAGTGCGCAGGATAAGTACCAGCACGGCAGCA
AAGATGACTTCTACATCAATCTCGGTTTAGCTGTACGTACCCTTCGCGAAGATTTGCCTC
TCCTCTTCACCAAAGATCTCAATTACGACATTTACAGGTGTGTTTTCCTCTTTCTCCTTT
AGGTGATTCCTTCCTTAAACCTGTCAATGAAAGCATTTGTGTTTCCTCTTTCAGGGATGA
TATAACATTAGTGGATCCAATGAACACGTTCTCTGGGATTGACAACTACAAATTGATCTT
CTGGGCACTTAGATTTCATGGCAAGATTCTCTTCAGGGATATCTCACTTGAGATCTTCAG
GGTTTGGCAACCATCAGAGAACATGATTCTCATCAGGTGGAATCTTAAAGGTGTACCTAG
AGTCCCATGGGAGGCAAAGGGAGAGTTTCAGGGTACTTCTCGCTATAAACTCGACCGAAA
TGGCAAAATCTATGAGCATAAGGTCGATAACTTGGCCTTCAACTTCCCTCACCAGCTCAA
ACCTGCCACTTCTGTTTTGGATATGGTCA

Last Updated on 06.12.2018 16:11