Sequences from confirmation amplicon


Confirmation sequences for line 236H07 and hit At1g27780 are shown.
Line ID 236H07
Locus At1g27780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:9682906
Primers used for PCR GD41 - 8474, show primer details
   
Sequence Name 39-K100814-0022-236-H07C-8409
GenBank Accession LN503277 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K100814-0022-236-H07C-8409
ATTTACAATTGAATATATCCTGTATTTATGTATTGACTATCAGGAGCTTTCCAAAACGTT
TGCAACCGCTGTTACCTCTGGTCGTGTGCCAGGCGTGTTAGTCAAATGCTATAGTTATTC
CATTCTACTTTATATGTTTGTTTATGTTATCCGCTATGTCATGTTTCAAATTATGATAAG
CCTTCTACTATGTTGTTTATGTTTATGTTGAAATTATATTATTGCTCAAGTTGAGAATTT
TTCAGCCAAATTCACATATGATATTTAGACACTGCAGTAAATGTAAATTTGGATATTAAA
ATCTAAGCGTCCACAACTCCGTACACGTGCATCTACCAAATAATACTTAGAGGTTTTTTC
TTTGTCTCGGAAAACTAGATATGGACGTTCACTAGCATGAGATATCAACTCTAACGTACA
CTAAAAAATAACACATGCAAACATATGAGGTCTTTGGTTTGTCTCATAAATGGTGATATT
TACGAATGAACGTACACTAATAGTACACTACAGCGTACACTGTCTGTTGAGTAAATGTAG
AAATGTAAATATGGACGTACACTAAAACGTACACTACAACGTACACTGTTTGTTGGGCAA
ATGTAAAAATGT
   
Sequence Name 39-K100826-0022-236-H07C-GD41
GenBank Accession LN503279 [GenBank]
Sequencing primer gene specific primer, GD41: 5'-TTACATTTTTACATTTGCCCAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K100826-0022-236-H07C-GD41
GTCCATATTTACATTTCTACATTTACTCAACAGACAGTGTACGCTGTAGTGTACTATTAG
TGTACGTTCATTCGTAAATATCACCATTTATGAGACAAACCAAAGACCTCATATGTTTGC
ATGTGTTATTTTTTAGTGTACGTTAGAGTTGATATCTCATGCTAGTGAACGTCCATATCT
AGTTTTCCGAGACAAAGAAAAAACCTCTAAGTATTATTTGGTAGATGCACGTGTACGGAG
TTGTGGACGCTTAGATTTTAATATCCAAATTTACATTTACTGCAGTGTCTAAATATCATA
TGTGAATTTGGCTGAAAAATTCTCAACTTGAGCAATAATATAATTTCAACATAAACATAA
ACAACATAGTAGAAGGCTTATCATAATTTGAAACATGACATAGCGGATAACATAAACAAA
CATATAAAGTAGAATGGAATAACTATAGCATTTGACTAACACGCCTGGCACACGACCAGA
GGTAACAGCGGTTGCAAACGTTTTGGAAAGCTCCTGATAGTCAATACATAAATACAGGAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCG

Last Updated on 06.12.2018 16:11