Sequences from confirmation amplicon


Confirmation sequences for line 237B10 and hit At4g04770 are shown.
Line ID 237B10
Locus At4g04770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:2429699
Primers used for PCR 13af - 8474, show primer details
   
Sequence Name 06-K106588-0022-237-B10C-8409
GenBank Accession LN488826 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K106588-0022-237-B10C-8409
GCATTTACATTGAATATATCCTGAAAACCCGAATCCTTAAAATTCCCATCTCCCAAATCC
CTAAATCCAACGAGACCGATCTTCAAACTCAGAGCCGATGTTGGAATCGATTCTCGTCCC
ATCGGAGCTTCTGAATCATCATCATCAGGTACATCAACCGTTAGCTCAACTGATAAACTT
CAACAGTATTTCCAAAATCTCGATTACGATAAGAAGTACGGATTCGTTGAAGATATCGAT
TCCTTCACAATCCCTAAAGGTTTATCTGAAGAGACGATTCGTTTGATTTCGAAATTGAAA
GAGGAACCAGATTGGATGCTTGAGTTTAGGTTCAAAG
   
Sequence Name 22-K106589-0022-237-B10C-13af
GenBank Accession LN496128 [GenBank]
Sequencing primer gene specific primer, 13af: 5'-CTTTGAACCTAAACTCAAGCATCC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K106589-0022-237-B10C-13af
AATAACGAATCGTCTCTTCGATAACCTTTAGGGATTGTGAAGGAATCGATCTCTTCAACG
AATCCGTACTTCTTATCGTAATCGAGATTTTGGAAATACTGTTGAAGTTTATCAGTTGAG
CTAACGGTTGATGTACCTGATGATGATGATTCAGAAGCTCCGATGGGACGAGAATCGATT
CCAACATCGGCTCTGAGTTTGAAGATCGGTCTCGTTGGATTTAGGGATTTGGGAGATGGG
AATTTTAAGGATTCGGGTTTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGATTATTA

Last Updated on 06.12.2018 16:11