Genotyping and primer details


Genotyping and primer details for 237B10 - At4g04770
Line ID 237B10
Locus At4g04770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:2429699 of line 237B10 Chr4:2429699
   
Insertion allele amplicon 8474 - 13af
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 13af: 5'-CTTTGAACCTAAACTCAAGCATCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:2429386
   
wt amplicon (over InsPos) 23sl - 13af
wt amplicon start Chr4:2429386
wt amplicon end Chr4:2430173
wt amplicon size 788 bp
Locus specific primer 13af: 5'-CTTTGAACCTAAACTCAAGCATCC-3'
2nd locus specific primer 23sl: 5'-TTAACAAAAACAAGAAACCAACCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11