Sequences from confirmation amplicon


Confirmation sequences for line 237C11 and hit At1g74670 are shown.
Line ID 237C11
Locus At1g74670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28053892
Primers used for PCR DB17 - 8474, show primer details
   
Sequence Name 17-K029910-022-237-C11C-8409
GenBank Accession LN493727 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K029910-022-237-C11C-8409
CATAAACTGATCCATATGGACATTTTGGTCCACCTTGTTGAGTCTTCCAGTTGTTGTAAC
AAGGACACACTTGTTTGTTGCCGTACGTGCCTGGAGGGACACAAAGGCATTTAGCACAAC
ACTTTTGGCAGAAGAACATGCATGGCTTATGATACTTTGTGTTGCTACATCTCCTTGTGC
ATTGTCCTCCACATTCTGCAATCATGATAATCGAGTTTTTATTTGATATATAATAAGAAA
CAAGATTCATAAAAGAAGAAGAAGTTTTTACTTACGGTATGATTTCAGACTTCCTGGTCC
ATACTGTTTAATTTCAAAACAAATTTTAACGCGGGTATTAGATTTATAGCAATAAATAGA
AAGGGAAAGGCAAAAACAATTGAATGAATTATAAAACTTACACTTTCTATTTTACCAAAA
AATAAAAACTTACACTTTCTGGATGGTACTCA
   
Sequence Name 17-K029910-022-237-C11C-DB17
GenBank Accession LN493728 [GenBank]
Sequencing primer gene specific primer, DB17: 5'-GTCAAAAGAAGCTGAGTACCATCC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K029910-022-237-C11C-DB17
AGTGTAGTTTTAAATTCATTCATTGTTTTTGCCTTTCCCTTTCTATTTATTGCTATAAAT
CTAATACCCGCGTTAAAATTTGTTTTGAAATTAAACAGTATGGACCAGGAAGTCTGAAAT
CATACCGTAAGTAAAAACTTCTTCTTCTTTTATGAATCTTGTTTCTTATTATATATCAAA
TAAAAACTCGATTATCATGATTGCAGAATGTGGAGGACAATGCACAAGGAGATGTAGCAA
CACAAAGTATCATAAGCCATGCATGTTCTTCTGCCAAAAGTGTTGTGCTAAATGCCTTTG
TGTCCCTCCAGGCACGTACGGCAACAAACAAGTGTGTCCTTGTTACAACAACTGGAAGAC
TCAACAAGGTGGACCAAAATGTCCATATGGATCAGTTTATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTT
TGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATT
CTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11