Sequences from confirmation amplicon


Confirmation sequences for line 237G04 and hit At5g45560 are shown.
Line ID 237G04
Locus At5g45560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18465820
Primers used for PCR GD39 - 8474, show primer details
   
Sequence Name 59-K100815-0022-237-G04C-8409
GenBank Accession LN509931 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K100815-0022-237-G04C-8409
CATTTCATAGAAAGAAAAAAAGTATGAGCTTGATAATGGATGATAAGGTTACTTGCTTAA
TAGATGCTTCTTTACATGTTCAATGTAGCTTCCTATCAAGACGATGGTTATTGATGGAAA
CTGTAGAGTTGAGGATCGTGGCTTGAAGACACATCATGGACATGTGAGTCTTATTATCTC
CTTGACTGTAATGTGAAACCTTTTTTTTTTTTGGGTTAAATTGAAATTTCATTTTGGCTT
TTGCAAGGTCCTTTTCTTCTGGACTTTTTTTTTGCAAAGGGTTTAGGGGTGGCCTTTTTA
AAACAAAAAAAAAAACCCCCCCAAATCCCGGGTTTTTGGGTTTTTTTTTCTAAAAAAAAA
TGGAAGAATTTTTATCTGGTACCAAACCTTTTAAAGGCTCCTGGTAATTTTATTGGGAGA
ATTTTTTTGCTAAAGG
   
Sequence Name 59-K100827-0022-237-G04C-GD39
GenBank Accession LN509933 [GenBank]
Sequencing primer gene specific primer, GD39: 5'-AAAGCTGCCATCTAGCAATAACAT-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K100827-0022-237-G04C-GD39
CAGAGCATAAATGTATAGTAACAAGATAAAACTACTACTATCTATTCTCAGAAAGAAAAC
ACAAGATACCGTGATTCTGTGGTGCTTTTCTTTCTTGTTATAAATAGACAACACATAAAC
CATCTGCAAAAAAAAAGTCAAGAAGATAAGTACATTGCAAATGCATAACTGAAATTACAA
TTAAACCAAAAAAAAAAAAGGGTTTCACTTTCCGGCCAGGGAAAAAAAAAAACCCAATTG
CCCTGAAGGGGTTTTAAACCCAAAATCCTAAATTTTACGTTTCCCATAAAAACCCTTCGT
TTGGATGGAAACCTCCTTGAAACTTGAAAAAAACCTTTTTTAAGGCAGGAACCCTTATCC
CCCTTTACCAGGCTCAAACTTTTTTTCTTTCTTTGGAAAAGGGCTCCAGGCCGGGAAAAT
CTCCGGGGATCACCATGGAGTATAAGGGCCAATTGGGAAAAAAAAAAAAAGAATTTCCCA
TTTTTTTTCCATTTCAAAAAGGAAAATTCCCCCCGCTTTTTAAAAAAAA

Last Updated on 06.12.2018 16:11