Genotyping and primer details


Genotyping and primer details for 237G04 - At5g45560
Line ID 237G04
Locus At5g45560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:18465820 of line 237G04 Chr5:18465820
   
Insertion allele amplicon 8474 - GD39
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer GD39: 5'-AAAGCTGCCATCTAGCAATAACAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:18466257
   
wt amplicon (over InsPos) 10jz - GD39
wt amplicon start Chr5:18465321
wt amplicon end Chr5:18466257
wt amplicon size 937 bp
Locus specific primer GD39: 5'-AAAGCTGCCATCTAGCAATAACAT-3'
2nd locus specific primer 10jz: 5'-TCTGTCAAAAGATGAATTTCGTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11