Sequences from confirmation amplicon


Confirmation sequences for line 237H05 and hit At5g28220 are shown.
Line ID 237H05
Locus At5g28220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:10191926
Primers used for PCR 09kw - 8474, show primer details
   
Sequence Name 58-K113845-0022-237-H05C-09kw
GenBank Accession LN509764 [GenBank]
Sequencing primer gene specific primer, 09kw: 5'-CGTGTGAAGCAACGTTTATAGTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K113845-0022-237-H05C-09kw
AACATTCTGGAACAAATAGTTTAACGTTATCTATACTTTTTATTACATAGCATCAATGCA
TTGTGATAGGAATTAAGTAGTATAAATGTTTCCGAAGAATGAAGGATTTGAGAAGTACAC
ATTCCAATTTGCCAAGTTATCTCATCGATTTCTTCCTTATGAACAATTTTTTTTCGTGTC
ACACACTTCATTCAACTTGTGAGAAATCGATGTTGATATCTTAAATGTCCATCAAACAAT
GATATCCGGATGAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGC
   
Sequence Name 31-K113844-0022-237-H05C-8409
GenBank Accession LN500244 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K113844-0022-237-H05C-8409
CCTTTACATTGAAATATTTCATCCGGATATCATTGTTTGATGGACATTTAAGAAATCAAC
ATCGATTTCTCACAAGTTGAATGAAGTGTGTGACACGAAAAAAAATTGTTCATAAGGAAG
AAATCGATGAGATAACTTGGCAAATTGGAATGTGTACTTCTCAATCCTTCATTCTTCGGA
AACATTTATACTACTTAATTCCTATCACAATGCATTGATGCTATGTAATAAAAAGTATAG
ATAACGTTAAACAATTTGGTTCCAAATGTTACATCCTTCAAGTCAACTCTAAGTTTTGAT
CTTGATATAAGATAATACACTATAAACGTGCTCTTCACAC

Last Updated on 06.12.2018 16:11