Sequences from confirmation amplicon


Confirmation sequences for line 238B07 and hit At1g76170 are shown.
Line ID 238B07
Locus At1g76170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28585870
Primers used for PCR IN15 - 8474, show primer details
   
Sequence Name 38-K033707-022-238-B07C-IN15
GenBank Accession LN502893 [GenBank]
Sequencing primer gene specific primer, IN15: 5'-TGTTGACAGTTAAAGTTGTTTCGG-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K033707-022-238-B07C-IN15
CAAGGATATATGACATCTCCTTCTCGTATGTATATTTGAAAGGTTTACATCTTGGAATTG
GACCATCTTCGCCTGTAGTGATTGATGTGCACCTACTCAATCTACAATAGCCAAATTACA
AATCAATCAATTAAGGGACATGAGACATATTATAGCCAAGATTCAAGGAAAAACATCACT
AGAAACGTATATATTTACCTAGCAATATCGCCTCGCAGTATGTTTAAGACAACTGTTTCG
GCTATATCATCTGCATTATGTCCAGTGACTAGCTTATCTACTTTCAATAACGCAGCTCCT
CGATCAAGTGCCTTCAAAAATTCAACCAGACATAGAATCATAAAACAGAAAAGACGGTTC
TGAAGAAACTTACTCAAAGAGGGAATGCTATGAAGAAACTTGCTCCTCACCTGTCGACGG
AATACACCACAGAAATGAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGAT
AAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAA
TTCGGAAATCTTTATTTCGACGTGTCTACATTCA
   
Sequence Name 38-K033707-022-238-B07C-oriL
GenBank Accession LN502894 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K033707-022-238-B07C-oriL
TTACAATTGAATATATTCATTTCTGTGGTGTATTCCGTCGACAGGTGAGGAGCAAGTTTC
TTCATAGCATTCCCTCTTTGAGTAAGTTTCTTCAGAACCGTCTTTTCTGTTTTATGATTC
TATGTCTGGTTGAATTTTTGAAGGCACTTGATCGAGGAGCTGCGTTATTGAAAGTAGATA
AGCTAGTCACTGGACATAATGCAGATGATATAGCCGAAACAGTTGTCTTAAACATACTGC
GAGGCGATATTGCTAGGTAAATATATACGTTTCTAGTGATGTTTTTCCTTGAATCTTGGC
TATAATATGTCTCATGTCCCTTAATTGATTGATTTGTAATTTGGCTATTGTAGATTGAGT
AGGTGCACATCAATCACTACAGGCGAAGATGGTCCAATTCCAAGATGTAAACCTTTCAAA
TATACATACGAGAAGGAGATTGTCATATATCCTTTGCTATTAAATCTTTTGTTTTGCTTC
CCGAAACAACTTTAACTGTCAAC
Primers used for PCR IN16 - 8474, show primer details
   
Sequence Name 39-K033707-022-238-B07C-8409
GenBank Accession LN503265 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K033707-022-238-B07C-8409
CCATTTACATTGAAGCTATAGCAACTCGTTCACCGAACTTAAACAAACGATTCCCAACAA
TGACTTGGTGAATCTCTTCCTCAAAAACCTCATAGAAACACTCTCTGCATATCTTTATAC
AAATACTCACATCATTATTGCTTATACGTAACAAACTAACTAGCAGGAGATTGAGAAACT
ATGATCTTTAACAGTTCCGAAAATGCCACAAGAGTAACGCAACCAAAGACCCAGCTCGAT
GATTTTGATCCTAATTCAAAATTTACCTGTTGAAGGGTTTTCGGTCGTTTTAGGACAGGT
CGTCACTGGTTACAGATGCAGCAGAGACGAGACCCTCCTGCTTTCTTGCTCTTCTCTTCC
CCTGCCTCCATCTCCACCGGTGAATGAAACTCCAAGTTTACTAGTCGGCGGTAAATTGTC
CTAACCGGAAAAACGACCTAACTCGGAGTTATATCCACAGAACACCGTCGTATTTGATTT
CGGTATCTGAATTGTTTTAGTTATTAATGGGCCCATTGGGCTTTCTTACCCAACATTAAA
ATCCCACATACAAGATTATAGATAATTAGATATACCCACAATGATGAGCGTTAAAGACAT
TATTAACTAAGGATGTGGTCCATTAATGGAAATGTTTTGTGATTAGCTGGAATCTCATG
   
Sequence Name 39-K033707-022-238-B07C-IN16
GenBank Accession LN503266 [GenBank]
Sequencing primer gene specific primer, IN16: 5'-CATGAGATCCAGCTAATCACAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K033707-022-238-B07C-IN16
CCTTAGTTAAATGTCTTTAACGCTCATCATTGTGGGTATATCTAATTATCTCTAATCTTG
TATGTGGGATTTTAATGTTGGGTAAGAAAGCCCAATGGGCCCATTAATAACTAAAACAAT
TCAGATACCGAAATCAAATACGACGGTGTTCTGTGGATATAACTCCGAGTTAGGTCGTTT
TTCCGGTTAGGACAATTTACCGCCGACTAGTAAACTTGGAGTTTCATTCACCGGTGGAGA
TGGAGGCAGGGGAAGAGAAGAGCAAGAAAGCAGGAGGGTCTCGTCTCTGCTGCATCTGTA
ACCAGTGACGACCTGTCCTAAAACGACCGAAAACCCTTCAACAGGTAAATTTTGAATTAG
GATCAAAATCATCGAGCTGGGTCTTTGGTTGCGTTACTCTTGTGGCATTTTCGGAACTGT
TAAAGATCATAGTTTCTCAATCTCCTGCTAGTTAGTTTGTTACGTATAAGCAATAATGAT
GTGAGTATTTGTATAAAGATATGCAGAGAGTGTTTCTATGAGGTTTTTGAGGAAGAGATT
CACCAAGTCATTGTTGGGAATCGTTTGTTTAAGTTCGGTGAACGAGTTGCTATAGCTTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTAT
TTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAGATCTTTATTTCGACGTGTCT
ACATTC

Last Updated on 06.12.2018 16:11