Sequences from confirmation amplicon


Confirmation sequences for line 239B04 and hit At5g59090 are shown.
Line ID 239B04
Locus At5g59090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23852897
Primers used for PCR CR13 - 8474, show primer details
   
Sequence Name 59-K017193-022-239-B04C-CR13
GenBank Accession LN509848 [GenBank]
Sequencing primer gene specific primer, CR13: 5'-CAGAGAAGGTACTATCGGTTCCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K017193-022-239-B04C-CR13
AATTTGGCCGACATTGAAGGTATAGTTAAGGTTTCTAGGCAAGATTTTGTTTTTCTTGGA
GCACTTGACGGTATCACCCGAGATGATTTTAAGGGTCTTCGAAGTGTAGTTCATGCCGCA
GAGAAAGGCGATGTGGTCAGCTTTGTCCAATTCATAGACAAGTCCAGGATTTAAAGCGGC
CATCGGGTCGACATGTCCAGCTCCATAAGCAAACTCGGTCGATGCGATGCCACGTCCCTT
AGCTTTTACAGGCCAAGCTGCAAAGATTAGTTAAGATAACAGTCAACAAAGGTTACTTGT
AGCACAAGAAAGTAAAAATTGCATTCAGTATTGCTTACCAGTTGTCATGATGGCAGATTG
AATCATGGAAGGAGACCATCTAGGGTAAAACGTCTTGACATACGCAGCCACCCCTGCAAC
ATGAGGACAAGCCATTGAAGTTCCAGAGAAAACAGAGTACTTCACACGTCTGGACAATAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGT
CGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACG
TGTCTACAT
   
Sequence Name 63-K017193-022-239-B04C-8409
GenBank Accession LN510872 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K017193-022-239-B04C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATTGTCCAGACGTGTGAA
GTACTCTGTTTTCTCTGGAACTTCAATGGCTTGTCCTCATGTTGCAGGGGTGGCTGCGTA
TGTCAAGACGTTTTACCCTAGATGGTCTCCTTCCATGATTCAATCTGCCATCATGACAAC
TGGTAAGCAATACTGAATGCAATTTTTACTTTCTTGTGCTACAAGTAACCTTTGTTGACT
GTTATCTTAACTAATCTTTGCAGCTTGGCCTGTAAAAGCTAAGGGACGTGGCATCGCATC
GACCGAGTTTGCTTATGGAGCTGGACATGTCGACCCGATGGCCGCTTTAAATCCTGGACT
TGTCTATGAATTGGACAAAGCTGACCACATCGCCTTTCTCTGCGGCATGAACTACACTTC
GAAGACCCTTAAAATCATCTCGGGTGATACCGTCAAGTGCTCCAAGAAAAACAAAATCTT
GCCTAGAAACCTTAACTATCCTTCAATGTCGGCCAAATTGTCAGGAACCGATAGT

Last Updated on 06.12.2018 16:11