Sequences from confirmation amplicon


Confirmation sequences for line 239H12 and hit At3g27320 are shown.
Line ID 239H12
Locus At3g27320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:10091665
Primers used for PCR 17hw - 8474, show primer details
   
Sequence Name 45-K112831-0022-239-H12C-17hw
GenBank Accession LN505589 [GenBank]
Sequencing primer gene specific primer, 17hw: 5'-AGAAGTAGGAATTTGCCTGTTTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K112831-0022-239-H12C-17hw
ACGGGTCATTAGGACTTGAGCCACAACCTTGACAGGATCTAGATTTTGACCAACTTCAAT
GGCTTTGCGGGCTACATAGTCTGCTATATTGGCACCACAGCTCACACCGAGAAGCACACA
TCTGAAGTTTCAAATTTCGAGAAGTCACAGAACTCAGTAAGATTTTTGGTCATATGAAAG
CAACGATCTACAAAATTTATCTATACTCAGCAAGTGTTGTTAAGAACCAAAAGCAGAACA
TTTATGGAATGCAAACTCCTTGAACTACGTGAAAGCAGTGATCATTTAAAAAATGAGAAA
GAAAAAAAAAAGACTTCTACATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCACGATTATAAAAAA
   
Sequence Name 16-K112829-0022-239-H12C-8409
GenBank Accession LN493501 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K112829-0022-239-H12C-8409
CTTTTTTTTTCTTTTCATTTTTAAATGATCACGTGCTTTCACGTAGTTCAAGGAGTTTGC
ATTCCATAAATGTTCTGCTTTTGGTTCTTAACAACACTTGCTGAGTATAGATAAATTTTG
TAAATCGTTGCTTTCATATGACCAAAAATCTTACTGAGTTCTGTGACTTCTCGAAATTTG
AAACTTCAAATGTGTGCTTCTCGGTGTGAGCTGTGGTGCCAATATAGCAAACTATGTAGC
CCGCAAAGCCATTGAAGTTGGTCAAAATCTAAATCCTGTCAAGGTTGTGGCTCAAGTCCT
AAGGTACCCGTTCTTCATCGGTAGCGTACCCACACAGTCGGAGATCAAACAGGCAAATTC
CTACTT

Last Updated on 06.12.2018 16:11