Sequences from confirmation amplicon


Confirmation sequences for line 240D03 and hit At5g51310 are shown.
Line ID 240D03
Locus At5g51310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20853028
Primers used for PCR PH28 - 8474, show primer details
   
Sequence Name 14-K017545-022-240-D03C-PH28
GenBank Accession LN492288 [GenBank]
Sequencing primer gene specific primer, PH28: 5'-CTTGTTTTCACCACTGACAAGGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K017545-022-240-D03C-PH28
TAATCTCCTCGATAGCATTACTAGTGAAAGTTATAAGAATATAACTTGAAACCAAATCAA
TTGTTTTTTTTCTTCGTCTTTTTGATCAATTTTTACAAAACCTTTCTACTGGTTTCTAAT
TTTATGCATTTTTATTATTTTTAATGATGTGTTGATGTTTTGGAAAAACAATTTGCATGA
ACACCAAACCAATTGTTTACATTGTTAACTAAATGATTTTGTTCCTTTCTCTTTTTTTTT
TAAAAAAGTGGGTTGATGAAAGAATATGGAGAAAAGATGACCAAACTATGTGAAAAAATA
ATGAAAGCAATCCTCTCTAGCTTCGGAAACGATCTTCATCACAAGTATTACCAATCTGAG
TTTGGAAACTGTCATGGCTACTTCAGGATCAACAACTACACAATCCCTTCAAACCAAGAA
AATGATCATCCCAACGGTGATGAGCAAAATTTGATCCAAGGGCTAGGAATGCACACTGAC
ATGAGTTGCATCACGATCGTTGACCAAAATGACATCGGAGGTCTTCAAGTCAAAACCAAA
AATGGGATTGGTCTGATGGATATCAACCCTAAAGATGAAGCACTCGTTGTCAACGTCGGA
GATCTATTGCATGCATGGACCAATGGACGG
   
Sequence Name 18-K017545-022-240-D03C-8409
GenBank Accession LN494101 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K017545-022-240-D03C-8409
TCCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCGTCAAAGCACCAA
AAAAAAGCAAGTGAGAATCTATTACCAACGAAACCACGTCGTTTCAAGATAACTCTGTGT
TGAGACGATCTCAGCCGTCCATTGGTCCATGCATGCAATAGATCTCCGACGTTGACAACG
AGTGCTTCATCTTTAGGGTTGATATCCATCAGACCAATCCCATCTCTGGTTCTGACTTGA
AGACCTCCGATGTCATCTTGGTCAACGATCGTGATGCAACTCATGTCAGTGTGCATTCCT
AGCCCTTCGATCAAATCTTGCTCATCACCGTTGTGATGATCATCTTCTTGGTCTGAAGGG
ATTGTGTAGTTGTTGATCCTGAAGTAGCCATGACAGTTTCCAAACTCAGATTCGTAATAC
TTGTGATGAAGATCGTCTCCGAAGCTAGAGAGGATTGCTTTCATTATCTTCTCACATAGT
TTCGTCATCTTTTCTCCATATTCTTTCATCAACCCACTTTTTTAAAAAAAAAAGAGAAAG
GAACAAAATCATTTAGTTAACAATGTAAACAATTGGTTTGGTGTTCATGCAAATTGTTTT
TCCAAAACATCAACACATCATTAAAAATAATAAAAATGCATAAAATTAGAAACCAGTAGA
AAGGTTTTGTAAAAATTGATCAAAAAGACGAAGAAAAAAAACAATTGATTTGGTTTCAAG
TATATTCTTATAAC

Last Updated on 06.12.2018 16:11