Sequences from confirmation amplicon


Confirmation sequences for line 240G11 and hit At2g34590 are shown.
Line ID 240G11
Locus At2g34590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14569757
Primers used for PCR TH20 - 8474, show primer details
   
Sequence Name 79-K103584-0022-240-G11C-TH20
GenBank Accession LN513817 [GenBank]
Sequencing primer gene specific primer, TH20: 5'-TCATTATCATGGCTGTTAATTGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K103584-0022-240-G11C-TH20
TTGTGATTGGCATTGCTCATCTATAGTGCATTAGATCTATGATACTGCTTCTGCTTGATT
GAATCTTGTGTTTTATTAGTAGTAGTTCATGTGAGGTTACGTATAATTTGTATCTACATC
CTCAAAGCCTACAAATTCAAGCGTATGCATTTTCACTTATGCATCCATTGTTCCAAGTAG
CACATTTCTTGCTCTTATCTCTGTATGTTCTGTGAGATATAAAACCTACTTCCATGTAAG
GTTTATGCCTTTTGCCACAGCATAAGCATAACTAAATTTAACTTGTGCAGACATGAGCTA
TTACTTTTCGAGGCTCTTCAAGAAGGTCTAGAAGAAGAGATGGACAGAGATCCACATGTA
TGTGTCATGGGTGAAGATGTAGGCCATTACGGAGGCTCATACAAAGTAACTAAAGGCCTA
GCTGATAAGTTTGGCGATCTCAGGGTTCTGGACACTCCAATCTGTGAAAACGCCTTCACC
GGTATGGGCATTGGAGCCGCCATGACAGGACTAAGACCCGTCATCGAAGGTATGAAATCT
ACATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GCA
   
Sequence Name 85-K103585-0022-240-G11C-8409
GenBank Accession LN514538 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K103585-0022-240-G11C-8409
ATTTACATGTAGATTTCATACCTTCGATGACGGGTCTTAGTCCTGTCATGGCGGCTCCAA
TGCCCATACCGGTGAAGGCGTTTTCACAGATTGGAGTGTCCAGAACCCTGAGATCGCCAA
ACTTATCAGCTAGGCCTTTAGTTACTTTGTATGAGCCTCCGTAATGGCCTACATCTTCAC
CCATGACACATACATGTGGATCTCTGTCCATCTCTTCTTCTAGACCTTCTTGAAGAGCCT
CGAAAAGTAATAGCTCATGTCTGCACAAGTTAAATTTAGTTATGCTTATGCTGTGGCAAA
AGGCATAAACCTTACATGGAAGTAGGTTTTATATCTCACAGAACATACAGAGATAAGAGC
AAGAAATGTGCTACTTGGAACAATGGATGCATAAGTGAAAATGCATACGCTTGAATTTGT
AGGCTTTGAGGATGTAGATACAAATTATACGTAACCTCACATGAACTACTACTAATAAAA
CACAAGATTCAATCAAGCAGAAGCAGTATCATAGATCTAATGCACTATAGATGAGCAATG
CCAATCACAATTAACTACCAAACTAGAGAGAACTTTTTCTTCCAATTAACAGCCTGGATA
AT

Last Updated on 06.12.2018 16:11