Sequences from confirmation amplicon


Confirmation sequences for line 241A07 and hit At1g06620 are shown.
Line ID 241A07
Locus At1g06620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2026046
Primers used for PCR 18fr - 8474, show primer details
   
Sequence Name 44-K111327-0022-241-A07C-18fr
GenBank Accession LN505222 [GenBank]
Sequencing primer gene specific primer, 18fr: 5'-CGGCTACTTTAACAAGTCCAAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K111327-0022-241-A07C-18fr
CATCGATCTCAAGGAGGTGGCACGGACTCGATCACGCGGCGGAGCTTGGTGGAGAAGATC
GGAGACGCGGCAGAGAAATGGGGATTTTTCCAGGTGATCAACCACGGGATCCCGATGGAT
GTGTTGGAGAAGATGATAGATGGGATTCGTGAGTTTCACGAGCAAGACACTGAAGTGAAG
AAAGGGTTTTACTCTCGAGATCCAGCTAGTAAGATGGTATACAGCAGCAACTTCGATCTG
TTTAGCTCACCGGCGGCGAATTGGAGAGATACACTTGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGCACTATATATTATAT
   
Sequence Name 36-K111326-0022-241-A07C-8409
GenBank Accession LN502268 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K111326-0022-241-A07C-8409
ATCGAAGTTGCTGCTGTATACCATCTTACTAGCTGGATCTCGAGAGTAAAACCCTTTCTT
CACTTCAGTGTCTTGCTCGTGAAACTCACGAATCCCATCTATCATCTTCTCCAACACATC
CATCGGGATCCCGTGGTTGATCACCTGGAAAAATCCCCATTTCTCTGCCGCGTCTCCGAT
CTTCTCCACCAAGCTCCGCCGCGTGATCGAGTCCGTGCCACCTCCTTTGAGATCGATTGT
GGGGATGGAGAAATCAGAGGAGGAAGGTGGTTTTGGACTTGTTAAAGTAGCCG

Last Updated on 06.12.2018 16:11